Characteristics of action of nitrosoalkylureas on cell level in winter wheat

  • M. M. Nazarenko Dnipropetrovsk State Agrarian and Economic University
Keywords: chromosomal aberrations, mutagen, wheat, genotype


Here we report the cytogenetic characteristics of variability of mutation induction in new wheat varieties and some relationships between the means of the cytogenetic characteristics and different doses and types of mutagens. Analysis of chromosomal aberrations caused by mutagenic action of any kind of mutagen by the meto-anaphase method is one of the best known and most precise methods which we can use for determining the fact of mutagenic action on plants, and identifying the nature of the mutagenic factor. The strategy of investigation combined the identification of genotypes with a specific low-sensitivity to mutagenic factors using cytological analysis screening of mutagen treated wheat populations with the approach of comparing different varieties by breeding methods to reveal their connections and differences, specific sensitivity to mutagenic effects on the cell level. Dry seeds of 8 varieties of winter wheat were treated by nitrosomethilurea at concentrations 0.0125%, 0.0250%, and nitrosoethilurea 0.010%, 0.025%, which are normal levels for mutagenic selection of winter wheat mutation. The frequency and spectra of chromosomal aberrations were investigated. The overall correlations between the frequency of chromosomal aberrations and the value of a concentration were at the level 0.7–0.8. It was established that the fragments-bridges ratio is a reliable parameter for identifyingthe nature of mutagen. We identified genotypes and groups of genotypes which have a resistance to different types of mutagenic action. The resistance of a group is dependant on the breeding method of the variety initially obtained. It expressed as a decrease in the frequency of aberrations. Varieties obtained through chemical mutagenesis were less sensitive to the same chemical mutagens. Higher rates of chromosomal aberrations were typical for varieties obtained through field hybridization without any mutagen treatment or when the initial material was changed by low temperatures. 


Adlera, I., Carereb, A., Eichenlaub-Ritterc, U., 2004. Gender differences in the induction of chromosomal aberrations and gene mutations in rodent germ cells. Environmental Research 17, 53–59. >>

Ahloowalia, B.S., Maluszynski, M., Nicoterlein, K., 2004. Global impact of mutation-derived varieties. Euphytica 135, 187–204. >>

Albokari, M., 2014. Induction of mutants in durum wheat using gamma irradiation. Pak. J. Bot. 46, 317–324.

Bignold, L.P., 2009. Mechanisms of clastogen-induced chromosomal aberrations: A critical review and description of a model based on failures of tethering of DNA strand ends to strand-breaking enzymes. Mutat. Res. 681, 271–298. >>

Geraskin, A.S., Dikareva, V.G., Dikareva, N.S., 2002. Vliyanie razdelnogo radioaktivnogo i himicheskogo zagryazneniya na vyihod tsitogeneticheskih narusheniy v interkalyarnoy meristeme yarovogo yachmenya [Effect of separation of radioactive and chemical pollution output cytogenetic damage in intercalary meristem spring barley]. Radiation Biology Radioecology 42, 364–368 (in Russian).

Grant, W.F., Owens, E.T., 2001. Chromosome aberration assays in Pisum for the study of environmental mutagens. Mutat. Res. 488, 93–118. >>

Hossain, M.F., Alam, M.S., 2001. Effect of gamma irradiation on the callus, developed from indica rise. Pak. J. Biol. Sci. 6, 670–671. >>

Huaili, Q., Lanming, X., Fei, H., 2005. Biological effect of the seeds of Arabidopsis thaliana irradiated by MeV protons. Radiat. Eff. Defect. S. 160, 131–136. >>

Karthika, I.R., Subba, B., 2006. Effect of gama rays and EMS on two varieties of soybean. Asian Journal of Biological Sciences 5, 721–724. >>

Klekka, W.R., 1989. Diskriminantnyiy analiz. Faktornyiy, diskriminantnyiy, klasternyiy analiz [Discriminant analysis. Factorial, discriminant, cluster analysis]. Finansy, Moscow (in Russian).

Korogodina, V.L., Panteleeva, A., Ganicheva, I., 1998. Vliyanie moschnosti dozyi gamma-oblucheniya na mitoz i adaptivnyiy otvet kletok pervichnyih korney prorostkov goroha [Effect of gamma irradiation on mitosis and adaptive response of cells of the primary roots of pea seedlings]. Radiation Biology Radioecology 38, 643–649 (in Russian).

Lifang, W., Zengliang, Y., 2001. Radiobiological effects of a low-energy ion beam on wheat. Radiat. Environ. Biophys. 40, 53–57. >>

Natarajan, A.T., 2005. Chromosome aberrations: Plants to human and feulgen to FISH. Curr. Sci. 89, 335–340. >>

Nazarenko, M., 2015. Negativnyie posledstviya mutagennogo vozdeystviya [Peculiarities of negative consequences of mutagen action]. Ecological Genetics 4, 25–26 (in Russian).

Nazarenko, M., 2015. Osobennosti vozdeystviya gamma-luchey na hromosomnyiy apparat kletki na primere pshenitsyi myagkoy ozimoy [The frequency and spectrum of chromosomal aberrations in root meristem cells of wheat under the action of mutagens]. Tambow State Unuversity Reports Natural and Technical Sciences 2, 449–452 (in Russian).

Rakhmatullina, E.M., Sanamyan, M.F., 2007. Estimation of efficiency of seed irradiation by thermal neutrons for inducing chromosomal aberration in M2 of cotton Gossypium hirsutum L. Russ. J. Genet. 43(5), 518–524. >>

Rank, J., Lopez, L.C., Nielsen, M.H., 2002. Genotoxicity of maleic hydrazide, acridine and DEHP in Allium cepa root cells performed by two different laboratories. Hereditas 136, 13–18. >>

Shu, Q.Y., Forster, B.P., Nakagawa, H. (ed.), 2011. Plant mutation breeding and biotechnology. FAO, Tokio. >>

Ukai, Y., 2006. Effectiveness and efficiency of mutagenic treatments: Gamma Field Symposia, 45, 1-4.

Van Harten, A.M., 1998. Mutation breeding. Theory and practical applications. Cambridge University Press, Cambridge. >>

Waugha, R., 2006. Harvesting the potential of induced biological diversity. Trends Plant Sci. 11, 71–79. >>