The production and analysis of carotenoid preparations from some strains of xylotrophic Basidiomycetes
AbstractThe aim of the study was selection of optimal conditions for obtaining carotenoid drugs of mycelium origin from the basidiomycete strains Laetiporus sulphureus Ls-08, Fomes fomentarius Ff-1201 and Fistulina hepatica Fh-18 and the study of antibacterial and total antioxidant activity of these compounds. The strains were surface grown on a glucose-peptone medium modified for each producer. The homogenized pigments of the mycelium strains were extracted with ethanol and the solvent was separated under vacuum at 60 ºC. The absorption spectra of the carotenoid drugs were recorded for alcoholic solutions at 350–500 nm. The antibacterial activity of the carotenoids was determined by the agar diffusion method, and the total antioxidant activity was determined by the DPPH-method. It was found that the optimum temperature for carotenoid extraction is 60 °C. The absorption spectra of carotenoid drugs showed three peaks in 420, 450 and 470 nm. These results respond to the β-carotene absorption spectra. The highest antioxidant activity was noted for carotenoid drugs from F. hepatica Fh-18 and L. sulphureus Ls-08 strains obtained at an extraction temperature of 40 and 60 °C respectively. The antibacterial activity of carotenoid drugs against the test cultures was not species dependent. Carotenoid drugs with a 20% concentrate obtained from the L. sulphureus Ls-08 strain had the highest antibacterial activity against the test cultures Staphylococcus aureus and Escherichia coli. Carotenoids from the mycelium of F. hepatica Fh-18 had the highest antibacterial activity against the test culture Candida albicans. Extraction temperature of 60 °C is optimal for mycelial yield of carotenoids from the studied strains. All preparations of carotenoids exhibited antibacterial activity against the test microorganism cultures. The carotenoid drugs obtained at 40 and 60 °C from the strains F. hepatica Fh-18 and L. sulphureus Ls-08 respectively showed the highest antioxidant activity.
Arvayo-Enrıquez, H., Mondaca-Fernandez, I., Gortarez-Moroyoqui, P., Lopez-Cervantes, J., Rodrıguez-Ramırez, R., 2013. Carotenoids extraction and quantification: A review. Analytical Methods 5(12), 2916–2925. >> doi.org/10.1039/c3ay26295b
Barry, A.L., 1993. Susceptibility tests: Diffusion test procedures. ASM Press, Washington D.C.
Britton, G., 1986. Biokhimiya prirodnykh pigmentov [Biochemistry of natural pigments]. Mir, Moskow (in Russian).
Bunghez, I.R., Avramescu, S.M., Neata, M., Radulescu, G., Ion, R., 2012. Obtaining of carotenoid extract from Lycium chinense and characterization using spectometrical analysis. Dig. J. Nanomater. Biostruct. 7(2), 523–528.
Cazzonelli, C.I., 2011. Carotenoids in nature: Insights from plants and beyond. Funct. Plant Biol. 38, 833–847. >> doi.org/10.1071/fp11192
Eldahshan, O.A., Singab, A.N., 2013. Carotenoids. J. Pharmacogn. Phytochem. 2(1), 225–234.
El-Rhman El-Banna, A.A., El-Razek, A.M., El-Mahdy, A.A., 2012. Isolation, identification and screening of carotenoidproducing strains of Rhodotorula glutinis. Food and Nutrition Sciences 3(5), 627–633. >> doi.org/10.4236/fns.2012.35086
Fedotov, O.V., 2007. Wooddestroying fungi as biosources of ferments for medicinal and nutritional purposes. Plant and microbial enzymes: Isolation, characterization and biotechnology applications. Myza, Tbilisi 125–131.
Fedotov, O.V., Veligodska, A.K., 2014. Search producers of polyphenols and some pigments among Basidiomycetes. Biotechnol. Acta 7(1), 110–116. >> doi.org/10.15407/biotech7.01.110
Fiedor, J., Burda, K., 2014. Potential role of carotenoids as antioxidants in human health and disease. Nutrients 4, 466–488. >> doi.org/10.3390/nu6020466
Gessler, N.N., Sokolov, A.B., Belozerskaya, T., 2003. Uchastiye ß-karotina v antioksidantnoy zashchite gribnoy kletki [The participation of ß-carotene in antioxidant protection of fungal cells]. Appl. Biochem. Microbiol. 39, 427–429 (in Russian).
Gómez-García, M.R., Ochoa-Alejo, N., 2013. Biochemistry and molecular biology of carotenoid biosynthesis in chili peppers (Capsicum spp.). Int. J. Mol. Sci. 14, 19025–19053. >> doi.org/10.3390/ijms140919025
Kapich, A.N., Gvozdkova, T.S., Kvacheva, Z.B., 2008. Antioksidantnyye, radiozashchitnyye i protivovirusnyye svoystva ekstraktov mitseliya griba Laetiporus sulphureus [Antioxidant, radioprotective and antiviral properties of the extracts of the mycelium fungus Laetiporus sulphureus]. Successes of Medical Mycology. US, Moscow. P. 146–148 (in Russian).
Molyneux, P., 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technolog. 26(2), 211–219.
Musienko, M.M., Parshikova, T.V., Slavniy, P.S., 2001. Spektrofotometricheskiye metody v praktike fiziologii, biokhimii i ekologii rasteniy [Spectrophotometric methods in the practice of physiology, biochemistry and ecology of plants]. Naukova Dumka, Kiev (in Russian).
Okawa, M., 2001. DPPH (1,1-Diphenyl-2-Picrylhydrazyl) radical scavenging activity of flavonoids obtained from some medical plants. Biol. Pharm. Bull. 24(10), 1202–1205. >> doi.org/10.1248/bpb.24.1202
Pintea, A., Bele, C., Andrei, S., Socaciu, C., 2003. HPLC analysis of carotenoids in four varieties of Calendula officinalis L. flowers. Acta Biologica Szegediensis 47, 37–40.
Pirog, T.P., 2010. Biotehnologia [Biotechnology]. NUHT, Kyiv (in Ukrainian).
Priseds’kiy, Y.G., 1999. Statystychna obrobka rezul’tativ biolohichnykh eksperymentiv [Statistical processing of biological experiments results]. Kassіopeya, Donetsk (in Ukrainian).
Regal, P., Amorim-Carrilho, K.T., Cepeda, A., Fente, C., 2014. Review of methods for analysis of carotenoids. Trends in Analytical Chemistry 56, 3–64. >>
Santos, M.F.G., Alves, R.E., Roca, M., 2015. Carotenoid composition in oils obtained from palm fruits from the Brazilian Amazon. Grasas Aceites 66(3), 43–51. >> doi.org/10.3989/gya.1062142
Veligodska, A.K., Fedotov, O.V., Petreeva, A.S., 2014. Vplyv dzherel azotnoho zhyvlennya na syntez karotynoyidiv deyakymy shtamamy bazydiomitsetiv [Effect of nitrogen nutrition sources on carotenoids synthesis for some basidiomycetes strains]. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University 4(1), 22–34 (in Ukrainian).
Wasser, S.P., 2010. Medicinal mushroom science: History, current status, future trends, and unsolved problems. Int. J. Med. Mush. 12(1), 1–16. >> doi.org/10.1615/IntJMedMushr.v12.i1.10