Optimum production conditions for the preparation of lemon balm (Melissa officinalis) tincture using response surface methodology


Keywords: tincture; bioactivity; optimization; aromatic plants; antiradical activity.

Abstract

Lemon balm (Melissa officinalis) is a member of medicinal and aromatic plants that have been used for phytotherapeutic applications for decades. In this study, optimum production conditions of lemon balm tincture, which is a functional product used commonly by the people for medicinal purposes, were investigated. For this aim, the response surface methodology approach was applied, and to determine the effect of processing variables, solid concentration (X1), ethanol concentration (X2), and processing time (X3) were selected as the parameters showing the essential effects on the tincture production. The production of high phenolic yielded tincture was aimed at, and therefore, total phenolic content (TPC) and antiradical activity as inhibition concentration (IC50) of the tinctures were determined as the responses in the study. According to the experimental design, 15 tincture samples were prepared, and TPC ranged 1,462–10,335 mg GAE/L, while the IC50 ranged 11.8–78.1 mL. Multiple response optimization approaches were expertly applied, and optimum solid concentration and ethanol concentration for the high bioactive lemon balm tincture was determined as 5 g in 30 mL solvent and 38.6%, respectively. These optimum production conditions will help manufacturing the ideal lemon balm tincture with high bioactivity.

References

Bone, K. (2003). A clinical guide to blending liquid herbs. Herbal Formulations for the individual patients. Churchill Livingstone, Elsevier, Missouri.


Bounihi, A., Hajjaj, G., Alnamer, R., Cherrah, Y., & Zellou, A. (2013). In vivo potential anti-inflammatory activity of Melissa officinalis L. essential oil. Advances in Pharmacological and Pharmaceutical Sciences, 2013, 101759.


Box, G. E. P., & Behnken, D. W. (1960). Some new three level designs for the study of quantitative variables. Technometrics, 7, 455–475.


Brighente, I. M. C., Dias, M., Verdi, L. G., & Pizzolatti, M. G. (2007). Antioxidant activity and total phenolic content of some Brazilian species. Pharmaceutical Biology, 45(2), 156–161.


Chan, E. W. C., Lim, Y. Y., Wong, S. K., Lim, K. K., Tan, S. P., Lianto, F. S., & Yong, M. Y. (2009). Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chemistry, 113(1), 166–172.


Hancianu, M., Aprotosoaie, A. C., Gille, E., Poiata, A., Tuchilus, C., Spac, A., & Stanescu, U. (2008). Chemical composition and in vitro antimicrobial activity of essential oil of Melissa officinalis L. from Romania. Revista Medico-Chirurgicala a Societatii de Medici si Naturalisti din Iasi, 12(3), 843–847.


He., B., Zhang, L. L., Yue, X. Y., Liang, J., Jiang, J., Gao, X. L., & Yue, P. X. (2016). Optimization of ultrasound assisted extraction of phenolic compounds and anthocyanins from blueberry (Vaccinium ashei) wine pomace. Food Chemistry, 204, 70–76.


Kaplan, M., Yilmaz, M. M., Köprü, S., Gözelle, H., Muhderem, G., & Uslu, R. (2018). Investigation of the change in yield and bioactivity of dandelion (Taraxacum officinale) tincture depending on liquid/solid ratio and ethanol concentration. Turkish Journal of Agricultural and Natural Sciences, 5(2), 163–174.


Kaplan, M., Yılmaz, M. M., Uslu, R., Köprü, S., Gözelle, H., & Muhderem, G. (2019). An optimization study for the production of Origanum onites tincture by response surface methodology: Effect of liquid/solid ratio, ethanol concentration and storage period. Tekirdağ Ziraat Fakültesi Dergisi, 16(1), 11–22.


Köprü, S., Uslu, R., Karaman, K., Yilmaz, M. M., & Kaplan, M. (2020). Optimization of processing parameters for the preparation of clove (Syzygium aromaticum) hydroalcoholic extract: A response surface methodology approach to characterize the biofunctional performance. Journal of Applied Research on Medicinal and Aromatic Plants, 16, 100236.


Kowalczyk, A., Biskup, I., & Fecka, I. (2012). Total phenolic content and antioxidative properties of commercial tinctures obtained from some Lamiaceae plants. Natural Product Communications, 7(12), 1631–1634.


Lara, M. S., Gutierrez, J. I., Timon, M., & Andres, A. I. (2011). Evaluation of two natural extracts (Rosmarinus officinalis L. and Melissa officinalis L.) as antioxidants in cooked pork patties packed in MAP. Meat Science, 88(3), 481–488.


Lin, J. T., Chen, Y. C., Lee, Y. C., Hou, C. W., Chen, F. L., & Yang, D. J. (2012). Antioxidant, anti-proliferative and cyclooxygenase-2 inhibitory activities of ethanolic extracts from lemon balm (Melissa officinalis L.) leaves. LWT-Food Science and Technology, 49, 1–7.


Ma, T., Wang, Q. H., & Wu, H. (2010). Optimization of extraction conditions for improving solubility of peanut protein concentrates by response surface methodology. LWT-Food Science and Technology, 43(9), 1450–1455.


Mimica-Dukic, N., Bozin, B., Sokovic, M., & Simin, N. (2004). Antimicrobial and antioxidant activities of Melissa officinalis L. (Lamiaceae) essential oil. Journal of Agricultural and Food Chemistry, 52(9), 2485–2489.


Mirolieai, M., Khazaei, S., Moshkelgosha, S., & Shirvani, M. (2011). Inhibitory effects of lemon balm (Melissa officinalis L.) extract on the formation of advanced glycation end products. Food Chemistry, 129, 267–271.


Nolkemper, S., Reichling, J., Stintzing, F. C., Carle, R., & Schnitzler, P., (2006). Antiviral effect of aqueous extracts from species of the Lamiaceae family against Herpes simplex virus type 1 and type 2 in vitro. Planta Medica, 72(15), 1378–1382.


Olech, M., Nowak, R., Los, R., Rzymowska, J., Malm, A., & Chrusciel, K. (2012). Biological activity and composition of teas and tinctures prepared from Rosa rugosa Thunb. Open Life Sciences, 7(1), 172–182.


Radomir, A. M., Guţă, I. C., Buciumeanu, E. C., & Pîrvu, M. L. (2019). Optimization of microwave extraction method of total polyphenols from lemon balm officinalis L. vitroplants. Studii şi Cercetări, 28(1), 112–118.


Salamon, I., Kryvtsova, M. V., Trush, K. I., Fandalyuk, A. I., & Spivak, M. J. (2019). Agro-ecological cultivation, secondary metabolite characteristics and microbiological tests of lemon balm (Melissa officinalis) – the variety Citronella. Regulatory Mechanisms in Biosystems, 10(2), 264–268.


Sanchez-Medina, A., Etheridge, C. J., Hawkes, G. E., Hylands, P. J., Pendry, B. A., Hughes, M. J., & Corcoran, O. (2007). Comparison of rosmarinic acid content in commercial tinctures produced from fresh and dried lemon balm (Melissa officinalis). Journal of Pharmacy and Pharmaceutical Sciences, 10(4), 455–463.


Sanchez-Moreno, C., Larrauri, J. A., & Saura-Calixto, F. (1998). A procedure to measure the antiradical efficiency of polyphenols. Journal of Agricultual and Food Chemistry, 47, 425–431.


Sheng, Z. L., Wan, P. F., Dong, C. L., & Li, Y. H. (2013). Optimization of total flavonoids content extracted from Flos populi using response surface methodology. Industrial Crops and Products, 43(1), 778–786.


Singleton, V. L., & Rossi Jr., J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. The American Journal of Enology and Viticulture, 16, 144–158.


Spiridon, I., Colceru, S., Anghel, N., Teaca, C. A., Bodirlau, R., & Armatu, A. (2011). Antioxidant capacity and total phenolic contents of oregano (Origanum vulgare), lavender (Lavandula angustifolia) and lemon balm (Melissa officinalis) from Romania. Natural Product Research, 25(17), 1657–1661.


Tang, H., & Choi, Y. H. (2013). Optimum extraction of bioactive alkaloid compounds from rhizome coptidis (Coptis chinese Franch.) using response surface methodology. Solvent Extraction Research and Development, 20, 91–104.


Thoo, Y. Y., Ho, S. K., Liang, J. Y., Ho, C. W., & Tan, C. P. (2010). Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia). Food Chemistry, 120(1), 290–295.


Vongsangnak, W., Gua, J., Chauvatcharin, S., & Zhong, J. J. (2004). Toward efficient extraction of notogingseng saponins from cultured cells of Panax notoginseng. Biochemical Engineering Journal, 18(2), 115–120.


Wang, X. S., Wu, Y. F., Chen, G. Y., Yue, W., Liang, Q. L., & Wu, Q. N. (2013). Optimization of ultrasound assisted extraction of phenolic compounds from Sparganii rhizoma with response surface methodology. Ultrasonics Sonochemistry, 20(3), 846–854.


Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., & Brygadyrenko, V. V. (2019). Antimicrobial activity of 50 plant extracts. Biosystems Diversity, 27(2), 163–169.


Zou, T. B., Wang, M., Gan, R. Y., & Ling, W. H. (2011). Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology. International Journal of Molecular Sciences, 12(5), 3006–3017.

Published
2020-05-14
Section
Articles