Effect of Capreolus capreolus and Sus scrofa excreta on alanine aminotransferase activity in Glechoma hederacea leaves in conditions of Cd pollution

  • O. M. Vasilyuk Oles Honchar Dnipropetrovsk National University
  • O. Y. Pakhomov Oles Honchar Dnipropetrovsk National University
Keywords: water-soluble protein fraction, heavy metal, mammals, maximum allowable concentration, anthropogenic stress


The paper reflects the analysis of Cd impact on the total activity (nM pyruvic acid/ml s) of alanine aminotransferase (ALT, EC nitrogen metabolism and the content (mg/ml) of water-soluble protein fraction (albumin) in Glechoma hederacea L. leaves subject, which dominated in the research area (natural floodplain oak with Stellaria holostea L.). Cd was introduced in the form of salts Cd(NO3)2 in the concentrations of 0.25, 1.25 and 2.50 g/m2, equivalent to Cd in 1, 5 and 10 doses of MAC. The content of doses of MAC of Cd (5 mg/kg soil) was taken into account during introduction. We observed the inhibition of the activity of ALT 3–4 times (with adding the Cd salts at a dose of 1 and 5 МAС) compared to controls (area without pollution of Cd and excreta of mammals). This stress reaction took place in the protein complex as well. Thus, albumin content was equal to 72% and 80% (with Cd 1 and 5 MAC) compared to control (the area without pollution and excretory functions of mammals). It proved nonspecific response to stress. Using excreta of Capreolus capreolus L. and Sus scrofa L. shows the reduction of Cd effects and increasing the metabolic activity of ALT by 41% and 105%, respectively (with adding of Cd 1 MAC) compared to control (pollution by Cd at a dose 1 MAC). The effect of Cd 5 MAC is offset (only with the introduction of C. capreolus excreta) compared to control (pollution by Cd at a dose 5 MAC). Normalization of the albumin content (with adding of Cd 1 and 5 MAC) compared to control (сontrol of Cd at a dose 1 MAC and сontrol of Cd at a dose 5 MAC) with using of excreta of C. capreolus was observed. In conditions of Cd at a doze 10 MAC the ALT activity was reduced 2 times with the introduction of excreta of C. capreolus as S. scrofa compared to control (Cd at a dose 10 MAC). The introduction of excreta of S. scrofa compared with C. capreolus restored the albumin content by 10% to the control. Thus, the feasibility of using different biological community representatives for comprehensive regulation of the environmental changes in the conditions of steppe Ukraine has been found.


Boshoff, М., Jonge, М.D., Scheifler, R., Bervoets, L., 2014. Predicting As, Cd, Cu, Pb and Zn levels in grasses (Agrostis sp. and Poa sp.) and stinging nettle (Urtica dioica) applying soil – plant transfer models. Sci. Total. Environ. 493(15), 862–871. >> doi: 10.1016/j.scitotenv.2014.06.076

Bradford, M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72, 248–254.

Dospekhov, B.A., 1985. Metodika polevogo opyta [Methods of experience of the field]. Agroprom Press, Moscow (in Russian).

Fuller-Espie, S.L., Bearoff, F.M., Minutillo, M.A., 2011. Exposure of coelomocytes from the earthworm Eisenia hortensis to Cu, Cd, and dimethylbenz[a]anthracene: An in vitro study examining reactive oxygen species production and immune response inhibition. Pedobiologia 54(29), S31–S36.

Hameed, A., Mahmooduzzafar, T.N.Q, Siddiqi, T.O., Iqbal, M., 2011. Differential activation of the enzymatic antioxidant system of Abelmoschus esculentus L. under CdCl2 and HgCl2 exposure. Braz. J. Plant Physiol. 23(1), 46–54.

Hasan, S.A., Hayat, S., Wani, A.S, Ahmad, A., 2011. Establishment of sensitive and resistant variety of tomato on the basis of photosynthesis and antioxidative enzymes in the presence of cobalt applied as shotgun approach. Braz. J. Plant Physiol. 23(3), 175–185.

Has-Schön, E., Bogut, I., Vuković, R., Galović, D., Bogut, A., Horvatić, J., 2015. Distribution and age-related bioaccumulation of lead (Pb), mercury (Hg), cadmium (Cd), and arsenic (As) in tissues of common carp (Cyprinus carpio) and European catfish (Sylurus glanis) from the Buško Blato reservoir (Bosnia and Herzegovina). Chemosphere 135, 289–296.

Joshi, G., Shukla, A., Shukla, A., 2011. Synergistic response of auxin and ethylene on physiology of Jatropha curcas L. Braz. J. Plant Physiol. 23(1), 66–77. >> doi: 10.1590/S1677-04202011000100009

Kobyletska, M., Terek, O., 2002. Vplyv ioniv kadmiiu na vmist fenolnykh spoluk ta vilnoho prolinu v roslynakh kukurudzy [The impact of cadmium ions on the content of phenolic compounds and free proline in maize plants]. Visn. Lviv. Univ. Ser. Biol. 28, 311–316.

Kuta, E., Jędrzejczyk-Korycińska, M., Cieślak, E., Rostański, A, Szczepaniak, M., Migdałek, G, Wąsowicz, P., Suda, J., Combik, М., Słomka, А., 2014. Morphological versus genetic diversity of Viola reichenbachiana and V. riviniana (sect. Viola, Violaceae) from soils differing in heavy metal content. Plant Biol. 16(5), 924–934.

Li, Z.H., Chen, L., Wu, Y.H., Li, P., 2014. Effects of mercury on oxidative sand gene expression of potential biomarkers in larvae of the Chinese rare minnow Gobiocypris rarus. Environ. Contam. Toxicol. 67(2), 245–251.

Páez-Osuna, F., 2015. Bioavailability of cadmium, copper, mercury, lead, and zinc in subtropical coastal lagoons from the southeast Gulf of California osing mangrove oysters (Crassostrea corteziensis and Crassostrea palmula). Environ. Contam. Toxicol. 68(2), 305–316.

Pakhomov, A.Y., Vasilyuk, O.M., 2012. Aktyvinst' fermentiv pereaminuvannya yak indykator seredovyshchetvirnoyi funktsiyi Mammalia v transformovanykh ekosystemakh [Аctivity of transamination enzymes as the indicator of environmantal forming function of Mammalia representatives in transformed antropogenic ecosystem]. Sci. Vis. Cher. Univ. Ser. Biol. System. 4(4), 455–462.

Polevoy, V., Maximov, G. (ed.), 1978. Metody biohimicheskogo analiza rastenij [Methods of analysis biochemically of plants]. Leningrad Univ. Press, Leningrad (in Russian).

Rau, S., Miersch, J., Neumann, D., Weber, E., Krauss, G.-J., 2007. Biochemical responses of the aquatic moss Fontinalis antipyretica to Cd, Cu, Pb and Zn determined by chlorophyll fluorescence and protein levels. Environ. Exp. Bot. 59(3), 299–306. >> doi.org/10.1016/j.envexpbot.2006.03.001

Shamim, S., Rehman, A., 2014. Cadmium-resistance mechanism in the bacteria Cupriavidus metallidurans CH34 and Pseudomonas putida mt2. Environ. Contam. Toxicol. 67(2), 149–157. >> doi.org/10.1007/s00244-014-0009-7

Song, Y.F., Luo, Z., Pan, Y.X., Liu, X., 2014. Effects of copper and cadmium on lipogenic metabolism and metal element composition in the javelin goby (Synechogobius hasta) after single and combined exposure. Environ. Contam. Toxicol. 67(2), 167–180. >> do: 10.1007/s00244-014-0011-0

Vasilyuk, O.M., Pakhomov, A.Y., 2012. Vplyvi ioniv nikelju na funkcional'nu aktyvnist' transaminaz v lystkah Glechoma hederatia L. v umovahryjnoi' aktyvnosti Mammalia [Effect of nickel ions on the functional activity of enzymes in the leaves of Glechoma hederatia L. in digging activity of Mammalia]. Achievement of High school-2012: Materialy VIІI Mezhdunarodnoj Nauchno-Prakticheskoj Konferencii. Bjal GRAD-BG, Sofija, 21, 43–49 (in Ukrainian).

Vasilyuk, O.M., Pakhomov, A.Y., 2014. Еffect of lead ions on alanine aminotransferase activity in Glechoma hederacea leaves subject. Scientific Enquiry in the Contemporary World: Theoretical basics and innovative approach. Colloquium, California, USA, 19–26.

Wang, J., Li, W., Zhang, C., Ke, S., 2010. Physiological responses and detoxific mechanisms to Pb, Zn, Cu and Cd in young seedlings of Paulownia fortunei. J. Environ. Sci. 22(12), 1916–1922. >> doi.org/10.1016/S1001-0742(09)60339-9

Weyens, N., Gielen, M., Beckers, B., Boulet, J., van der Lelie, D., Taghavi, S., Carleer, R., Vangronsveld, J., 2014. Bacteria associated with yellow lupine grown on a metal-contaminated soil: In vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction. Plant Biol. 16(5), 998–996.

Wu, F.Y., Bi, Y.L., Leung, H.M., Ye, Z.H., Lin, X.G., Wong, M.H., 2010. Accumulation of As, Pb, Zn, Cd and Cu and arbuscular mycorrhizal status in populations of Cynodon dactylon grown on metal-contaminated soils. Appl. Soil Ecol. 44(3), 213–218. >> doi: 10.1016/j.apsoil.2009.12.008