Chlorella sphaerica and its position in the Genus diplosphaera (Chlorophyta, Trebouxiophyceae)


  • V. M. Karbovska Taras Shevchenko Kyiv National University
  • I. Y. Kostikov Taras Shevchenko Kyiv National University

Abstract

Morophological and molecular genetic analyses of green alga Chlorella sphaerica Tschermak-Woess (authentic strain), which represents the taxonomically critical species, the phenotypic similarity and phylogenetic close relation with the genus Diplosphaera Bialosuknia were foundbutthe significant difference from the genus Chlorella Bejerink was demonstrated. Discovery of new morphological features (presence of vegetative division, the ability to dyads and packages forming, as well as external mucous structures) indicates the position of Ch. sphaerica within the genus Diplosphaera. The analysis of nucleotide sequences of non-coding regions (ITS-1 and ITS-2) of nuclear-encoding ribosomal gene cluster confirms this conclusion. Based on these data the new nomenclature combination is suggested: Diplosphaera sphaerica (Tschermak-Woess) Karbovska et Kostikov, comb. nova (basionym: Chlorella sphaerica Tschermak-Woess). 

References

Масюк Н. П. Эволюционные аспекты морфологии эукариотических водорослей. – К. : Наук. думка, 1993. – 256 с.
A new aerial alga Stichococcus ampulliformis sp. nov. (Trebouxiophyceae, Chlorophyta) from Japan / S. Handa, M. Nakahara, H. Tsubota et al. // Phycological Research. – 2003. – Vol. 51. – P. 203–210. >> doi.:10.1111/j.1440-1835.2003.tb00188.x
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics / T. J. White, T. Bruns, S. Lee, J. Taylor // PCR protocols: A guide to methods and applications / Ed. M. A. Innis, D. H. Geifand, J. J. Snisky, T. J. White. – San Diego (CA), 1990. – P. 315–322.
Bialisuknia W. Diplosphaera chodati Bial. // Bull. de la Soc. bot. de Geneve. – 1909. – II, serie I. – 103 p.
Bischoff H. W. Some soil algae from Enchanted Rock and related algal species / H. W. Bischoff, H. C. Bold // Phycol. Stud. 4, Univ. Texas Publ. – 1963. – N 6318. – P. 1–95.
Broady P. A. New records of chlorophycean microalgae cultured from Antarctic terrestrial habitats // Nova Hedwigia. – 1982. – Vol. 36. – P. 445–484.
Ellias M. Pseudomarvania, gen. nov. (Chlorophyta, Trebouxiophyceae), a new genus for “budding” subaerial green algae Marvania aerophytica Neustupa et Sejnohova and Stichococcus ampulliformis Handa / M. Ellias, J. Neustupa // Fottea. – 2009. – Vol. 9, N 2. – P. 169–177.
Ettl H. Syllabus der Boden-, Luft und Flechtenalgen / H. Ettl, G. Gartner. – Gustav Fischer Verlag, Stuttgart, Jena, Germany, 1995. – 721 p.
Friedl T. Phylogenetic relationships of green algae assigned to the genus Planophila / T. Friedl, C. J. O'Kelly // Eur. J. Phycol. – 2002. – Vol. 37. – P. 373–384. >> doi.:10.1017/S0967026202003712
Identification of photobionts from the lichen family Physciaceae using algal-specific ITS rDNA sequencing / G. Helms, T. Friedl, G. Rambold, H. Mayehofer // The Lichenologist. – 2001. – Vol. 33. – P. 73–86. >> doi.:10.1006/lich.2000.0298
Johansen J. R. Recognizing cyanobacterial diversity through adoption of a new species paradigm / J. R. Johansen, D. A. Casamatta // Algol. Stud. – 2005. – Vol. 116. – P. 71–93. >> doi.:10.1127/1864-1318/2005/0117-0071
Nеustupa J. A taxonomic study of two Stichococcus species (Trebouxiophyceae, Chlorophyta) with a starch-enveloped pyrenoid / J. Nеustupa, M. Elias, L. Segnohova // Nova Hedwigia. – 2007. – Vol. 84, N 1–2. – P. 51–63. >> doi.:10.1127/0029-5035/2007/0084-0051
Pickett-Heaps J. Cell division in Stichococcus // European Journal of Phycology. – 1974. – Vol. 9, N 1. – P. 63–73. >> doi.:10.1080/00071617400650081
Testing for endemism, genotypic diversity and species concepts in Antarctic terrestrial microalgae of the Tribonemataceae (Stramenopiles, Xanthophyceae) / N. Rybalka, R. A. Andersen, I. Kostikov et al. // Environmental Microbiology. – 2009. – Vol. 11, N 3. – P. 554–565. >> doi.:10.1111/j.1462-2920.2008.01787.x
Tschermak-Woess E. New and known taxa of Chlorella (Chlorophyceae): Occurrence as lichen phycobionts and observations on living dictyosomes // P1ant Systematics and Evolution. – 1988. – Vol. 159. – P. 123–139. >> doi.:10.1007/BF00937430
Vischer W. Reproduktion und systematische Stellung einiger Rinden- und Bodenalgen // Schweiz. Zeitschr. Hydrol. – 1960. – Vol. 22, N 1. – Р. 330–349.
Published
2012-03-06
Section
Articles