Culture of isolated embryo sacs as a biotechnological system for the upgrowing of maize zygotic embryos in vitro


  • Е. V. Lyapustina Ukrainian State University of Chemical Engineering
  • T. M. Satarova Agricultural Steppe Zone Institute of the National Academy of Agrarian Sciences of Ukraine

Abstract

Our research established the possibility of updevelopment of maize embryo in a culture of isolated embryo sacs in artificial nutrient medium in vitro from the transition stage up to the beginning of organogenesis. It was determined that the cultivation of embryo sacs for the purpose of an embryo upgrowing and starch accumulation in endosperm should continue more than 20 days. The colour of cultivated embryo sac can be considered as a marker of embryo’s upgrow and starch accumulation in endosperm.

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Published
2009-11-30
Section
Articles