Influence of the long-term cold on isolated maize immature embryos in vitro


  • T. M. Satarova Agricultural Steppe Zone Institute of the National Academy of Agrarian Sciences of Ukraine
  • D. E. Strunin The Institute of Grain Farming of UAAS,
  • P. N. Galushchak Ukrainian State University of Chemical Technology

Abstract

The influence of the long-term effect of low positive temperature on isolated immature embryos of different maize genotypes during their in vitro maintenance was studied. The possibility of obtaining vigorous green seedlings after 3-month cold storage of embryos was shown. The abilities of embryos of different lines and F2hybrids to germinate under the given conditions are compared. Perspectives of further investigations to ascertain the conditions of effective maintenance of isolated maize immature embryos and further obtaining vigorous seedlings are discussed.

References

Лакин Г. Ф. Биометрия. – М.: Высшая школа, 1990. – 352 с.
Сатарова Т. Н. Влияние различных факторов на эмбриокультуру кукурузы // Вісник аграрної науки. – 2002. – № 4. – С. 51–53.
Сатарова Т. М. Андрогенез та ембріокультура у кукурудзи in vitro. – Дис. ... д-ра біол. наук: 03.00.20 – біотехнологія. – К., 2002. – 537 с.
Сатарова Т. Н. Ускорение селекционного процесса кукурузы с помощью метода эмбриокультуры / Т. Н. Сатарова, Г. Р. Пиралов, Б. В. Дзюбецкий // Вісник аграрної науки. – 1993. – № 8. – С. 50–53.
Mol R. Embryogenesis and plant regeneration from maize zygotes by in vitro culture of fertilized embryo sacs / R. Mol, E. Matthys-Rochon, C. Dumas // Plant Cell Rep. – 1995. – Vol. 14. – P. 743–747. >> doi:10.1007/BF00232914
Van Lammeren A. A. M. Development morphology and cytology of the young maize embryo (Zea mays L.) // Acta Bot. Neerl. – 1986. – Vol. 35. – P. 169–188.
Van Lammeren A. A. M. Observations on the structural development of immature maize embryos (Zea mays L.) during in vitro culture in the presence and absence of 2,4 D // Acta Bot. Neerl. – 1988. – Vol. 37. – P. 49–61.
Published
2006-12-04
Section
Articles