Changes in the viability of the eggs of Ascaris suum under the influence of flavourings and source materials approved for use in and on foods


  • A. A. Boyko Dnipro State Agrarian-Economic University
  • V. V. Brygadyrenko Oles Honchar Dnipro National University http://orcid.org/0000-0002-9448-8232
Keywords: nematodes, antiparasitic activity, flavouring agents, cinnamaldehyde, benzoic acid, methylparaben

Abstract

One of the most common parasitic diseases of pigs globally is ascariasis. It is caused by the nematode Ascaris suum Goeze, 1782 (Nematoda, Ascaridata), which parasitises the small intestine of pigs in its mature form and the respiratory system at the larval stage. This helminthiasis causes immense damage to swine-rearing. Control of the ascariasis pathogen in the host’s organism and in the environment is essential for the health of the animals and successful swine-rearing. The results of studying the effect of flavourings and source materials approved for use in and on foods – cinnamaldehyde (0656 Codex Alimentarius), benzoic acid (Е210 Codex Alimentarius) and methylparaben (Е218 Codex Alimentarius) , on the viability of invasive eggs of A. suum are useful for determining the minimum concentration of solution of these substances for use against eggs of A. suum (10 g/l). The lowest efficiency against invasive eggs of A. suum was obtained for methylparaben (LD50 = 3850 ± 2130 mg/l), the highest was obtained with cinnamaldehyde (LD50 = 2437 ± 864 mg/l), and benzoic acid (LD50 = 1240 ± 680 mg/l). 

References

Antipov, N. (2010). Askaroz svynej [Ascariasis of pigs]. Propozycija, 12, 124–126 (in Ukrainian).
Araújo, J. V., Braga, F. R., Silva, A. R., Araujo, J. M., & Tavela, A. O. (2008). In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense, Pochonia chlamydosporia on Ascaris suum eggs. Parasitology Research, 102, 787–790.
Artemenko, L. P. (2011). Askaroz – nebezpechna invazija u svynej [Ascariasis – harmful infestation of pigs]. Suchasna Veterynarna Medycyna, 4, 48–52 (in Ukrainian).
Belletti, N., Kamdem, S. S., Tabanelli, G., Lanciotti, R., & Gardini, F. (2010). Modeling of combined effects of citral, linalool and β-pinene used against Saccharomyces cerevisiae in citrus-based beverages subjected to a mild heat treatment. International Journal of Food Microbiology, 136(3), 283–289.
Belœil, P. A., Chauvin, C., Fablet, C., Jolly, J. P., Eveno, E., Madec, F., & Reperant, J. M. (2003). Helminth control practices and infections in growing pigs in France. Livestock Production Science, 81, 99–104.
Bila, I. D. (1999). Pnevmonii' svynej, sprychyneni askaryso-mikrobnymy asociacijamy ta rozrobka profilaktychnyh i likuval'nyh zahodiv [Pneumonias of pigs caused by ascaris-microbe associations and development of prevention and treatment measures]. Instytut Eksperymental'noi' i Klinichnoi' Veterynarnoi' Medycyny, Kharkiv (in Ukrainian).
Boyko, A. A., & Brygadyrenko, V. V. (2017). Changes in the viability of Strongyloides ransomi larvae (Nematoda, Rhabditida) under the influence of synthetic flavourings. Regulatory Mechanisms in Biosystems, 8(1), 36–40.
Chiang, L.-C., Ng, L.-T., Cheng, P.-W., Chiang, W., & Lin, C.-C. (2005). Antiviral activities of extracts and selected pure constituents of Ocimum basilicum. Clinical and Experimental Pharmacology and Physiology, 32(10), 811–816.
De Velásquez, M. T. O., Martínez, J. L., Monje–Ramírez, I., & Rojas-Valencia, M. N. (2004). Destruction of helminth (Ascaris suum) eggs by ozone. Ozone: Science and Engineering, 26, 359–366.
Evstafieva, V. O. (2010). Asociatyvni invazii' svynej v umovah Lisostepu i Stepu Ukrai'ny [Associative infestations of pigs in the conditions of forest-steppe and steppe of Ukraine]. Nacional'nyj Universytet Bioresursiv i Pryrodokorystuvannja Ukrai'ny, Kyiv (in Ukrainian).
Ferreira, S. R., Araújo, J. V., Braga, F. R., Araujo, J. M., Carvalho, R. O., Silva, A. R., Frassy, L. N., & Freitas, L. G. (2011). Ovicidal activity of seven Pochonia chlamydosporia fungal isolates on Ascaris suum eggs. Tropical Animal Health and Production, 43, 639–642.
Feshenko, D. V. (2008). Osoblyvosti epizootologii', patogenezu ta terapii' zmishanoi' nematodoznoi' invazii' svynej [Peculiarities of epizootiology, pathogenesis and therapy of mixed nematodal infestation of pigs]. Veterynarna Medycyna Ukrai'ny, 2, 18–20 (in Ukrainian).
Fidjeland, J., Nordin, A., Pecson, B. M., Nelson, K. L., & Vinneras, B. (2015). Modeling the inactivation of ascaris eggs as a function of ammonia concentration and temperature. Water Research, 83, 153–160.
Fthenakis, G. C., Papadopoulos, E., & Himonas, C. (2005). Effects of three anthelmintic regimes on milk yield of ewes and growth of lambs. Veterinary Medicine, 52, 78–82.
Gudkova, A. Y., Petrov, Y. F., & Ivaniuk, V. P. (2006). Formirovanie parazitarnoj sistemy v organizme svinej pri nematodozah [Formation of the parasitic system in the organism of pigs with nematodes]. Veterinarija, 9, 31–33 (in Russian).
Kaplan, R. M., Storey, B. E., Vidyashankar, A. N., Bissinger, B. W., Mitchell, S. M., Howell, S. B., Mason, M. E., Lee, M. D., Pedroso, A. A., Akashe, A., & Skrypec, D. J. (2014). Antiparasitic efficacy of a novel plant-based functional food using an Ascaris suum model in pigs. Acta Tropica, 139, 15–22.
Katakam, K. K., Mejer, H., Dalsgaard, A., Kyvsgaard, N. C., & Thamsborg, S. M. (2014). Survival of Ascaris suum and Ascaridia galli eggs in liqui manure at different ammonia concentrations and temperatures. Veterinary Parasitology, 204, 249–257.
Katakam, K. K., Thamsborg, S. M., Dalsgaard, A., Kyvsgaard, N. C., & Mejer, H. (2016). Environmental contamination and transmission of Ascaris suum in Danish organic pig farms. Parasites and Vectors, 9, 80.
Katakam, K. K., Thamsborg, S. M., Kyvsgaard, N. C., Dalsgaard, A., & Mejer, H. (2014). Development and survival of Ascaris suum eggs in deep litter of pigs. Parasitology, 141, 1646–1656.
Kim, M.-K., Pyo, K.-H., Hwang, Y.-S., Park, K. H., Hwang, I. G., Chai, J.-Y, & Shin, E.-H. (2012). Effect of temperature on embryonation of Ascaris suum eggs in an environmental chamber. The Korean Journal of Parasitology, 50(3), 239–242.
Nelson, K. L., & Darby, J. L. (2001). Inactivation of viable Ascaris eggs by reagents during enumeration. Applied and Environmental Microbiology, 67(12), 5453–5459.
Nordin, A., Nyberg, K., & Vinneras, B. (2009). Inactivation of Ascaris eggs in source-separated urine and feces by ammonia at ambient temperatures. Applied and Environmental Microbiology, 75(3), 662–667.
Pedersen, S., Saeed, I., Friis, H., & Michaelsen, K. F. (2001). Effect of iron deficiency on Trichuris suis and Ascaris suum infections in pigs. Parasitology, 122(5), 589–598.
Pedersen, S., Saeed, I., Michaelsen, K. F., & Friis, H. (2002). Impact of protein energy malnutrition on Trichuris suis infection in pigs concomitantly infected with Ascaris suum. Parasitology, 124(5), 561–568.
Pereckiene, A., Kaziunaite, V., Vysniauskas, A., Petkevicius, S., Malakauskas, A., Sarkunas, M., & Taylor, M. A. (2007). A comparison of modifications of the McMaster method for the enumeration of Ascaris suum eggs in pig faecal samples. Veterinary Parasitology, 149, 111–116.
Ponomar, S. I., Soroka, N. M. (2008). Gel'mintogematologichni ta gel'minto-mamologichni doslidzhennja pry strongiloi'dozi svynej [Helminth-hemato-logical and helminth-mammalogical studies of pigs with strongyloidosis]. Naukovyj Visnyk Nacional'nogo Agrarnogo Universytetu, 7, 233–240 (in Ukrainian).
Rojas-Oropeza, M., Hernández-Uresti, A. S., Ortega-Charleston, L. S., & Cabirol, N. (2016). Effect of volatile fatty acids in anaerobic conditions on viability of helminth ova (Ascaris suum) in sanitization of municipal sludge. Environmental Technology, in press.
Sato, K., Krist, S., & Buchbauer, G. (2006). Antimicrobial effect of trans-Cinnamaldehyde, (–)-Perillaldehyde, (–)-Citronellal, Citral, Eugenol and Carvacrol on airborne microbes using an airwasher. Biological and Pharmaceutical Bulletin, 29(11), 2292–2294.
Si, W., Ni, X., Gong, J., Yu, H., Tsao, R., Han, Y., & Chambers, J. R. (2009). Antimicrobial activity of essential oils and structurally related synthetic food additives towards Clostridium perfringens. Journal of Applied Microbiology, 106(1), 213–220.
Somolinos, M., Garcia, D., Pagan, R., & Mackey, B. (2008). Relationship between sublethal injury and microbial inactivation by the combination of high hydrostatic pressure and citral or tert-butyl hydroquinone. Applied and Environmental Microbiology, 74(24), 7570–7577.
Veneziano, V., Rubino, R., Fedele, V., Rinaldi, L., Santaniello, M., Schioppi, M., Cascone, C., Pizzillo, M., & Cringoli, G. (2004). The effects of five anthelmintic treatment regimes on milk production in goats naturally infected by gastrointestinal nematodes. South African Journal of Animal Science, 34, 238–240.
Williams, A., Pena-Espinoza, M., Hansen, T. A., Boas, U., & Thamsborg, S. M. (2015). Purified extracts from chicory (Cichorium intybus) inhibit Ascaris suum glutathione-S-transferase activity and reduce survival of larvae in vitro. 25th International Conference of the World Association for the Advancement of Veterinary Parasitology: Abstract Book. Liverpool, United Kingdom. P. 142–142.
Zajac, A. M., & Conboy, G. A. (eds.) (2011). Veterinary clinical parasitology, 8th ed. John Wiley and Sons, UK
Published
2017-05-17
Section
Articles