Studying the genetic structure of Quercus robur forest stands on anthropogenically transformed territories using introns of the β-tubulin gene


  • Y. V. Pirko Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine
  • A. E. Demkovich Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine
  • L. O. Kalafat Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine
  • Y. B. Blume Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine
  • О. А. Lykholat University of Customs and Finance
Keywords: spatial structure; molecular markers; polymerase chain reaction

Abstract

Based on the analysis of the intron polymorphism of β-tubulin genes, the genetic variability of old Quercus robur L. trees from “Holosiivsky” NPP was investigated. The genotyping of 55 old Q. robur trees was carried out; 40 polymorphic and one monomorphic (about 880 bp) TBR fragments were found. High frequency (70–90%) of occurrence of fragments with an approximate molecular weight of 275, 490, 500, and 1110 bp was observed.The genetic polymorphism of old Q. robur trees was assessed as quite high: РІС is 0.22 – 0.39, the effective number of alleles per locus was 1.174–1.268. The Shannon information index was in the range of 0.204–0.269.The geographical differentiation of the genetic structure of centuries-old oak trees from “Holosiivsky” NPP was not pronounced. The share of inter-selection genetic variability (AMOVA) accounts for about 6% of genetic variability, and the geographic component – about 1%. Around 93% of genetic variability is concentrated on the individual level. Using the ТВР method, we found that Q. robur forest stands do not have a stabilized genetic and visible spatial structure, but at the same time they possess a sufficiently large genetic diversity. The absence of a spatial genetic structure may indicate the artificial origin of Q. robur trees from different seed materials, and also that a small number of the plants have survived to this time. In this case, the main influence on the structure of oak stands in “Holosiivsky” NPP was from anthropogenic factors, both in the form of cutting down trees and, possibly, the introduction of alien seed material.

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2018-11-10
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