The lipid peroxidation intensity of fungi strains from the orders Agaricales and Polyporales
AbstractThis article is devoted to investigation of the dynamics of growth and level of spontaneous and induced lipid peroxidation intensity of Basidiomycetes strains grown by surface cultivation on a glucose-peptone medium. The materials of the research are mycelium and culture filtrates (CF) of 57 strains (5 belong to 5 species from the order Polyporales s.l., and 52 belong to 7 species of the order Agaricales s.l.). To study the dynamics of growth we used a weighing method for determining the accumulation of absolutely dry biomass. Intensity of lipid peroxidation was determined by a modified spectrophotometric method for content of active to thiobarbituric acid products. It was found that the most productive in absolutely dry biomass accumulation were the strains Flammulina velutipes (Curt.: Fr.) Sing. F-610 and Pleurotus eryngii (DC.: Fr.) Quél. P-er. The level of spontaneous and induced LPO intensity in mycelia of all strains was higher than this figure in the culture filtrate and increased with the duration of cultivation. Dependencies between the content of lipid peroxidation products in the mycelia and CF were not established. The lowest values were recorded for biomass accumulation by the strains Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. P-14, P-192 and P. citrinopileatus Singer. Р-сіtr. Groups of basidiomycete cultures with different levels of TBA-AP were identified. Spontaneous and induced intensivity of lipid peroxidation in all studied strains of mycelia was higher than the figure in the culture filtrate. The intensity of lipid peroxidation in both mycelia and culture filtrate constantly increased, which can be explained by the growing shortage of certain nutrients (primarily carbon) and increased concentration of metabolic products in the medium. The ratio of spontaneous and induced lipid peroxidation intensity is specific to each strain and is independent of its systematic position. Shifting of prooxidant-antioxidant balance to a relatively stationary level is a mark of stress reaction. LPO-products can be both inductors and primary mediators of stress as a special class of biological systems. Selected strains with high rates of growth and LPO ntensity are promising for applications in biotechnology and ecology.
Baraboy, V.A., Orel, V.E., Karnaukh, I.M., 1991. Perekisnoye okisleniye i radiatsiya [Lipid peroxidation and radiation]. Naukova Dumka, Kiev (in Russian).
Belozerskaya, T.A., Gessler, N.N., 2007. Aktivnyye formy kisloroda i strategiya antioksidantnoy zashchity u gribov [Reactive oxygen species and antioxidant defense strategy in fungi]. Prikladnaya Biokhimiya i Mikrobiologiya 43(5), 565–575 (in Russian).
Chang, S.T., 2001. A 40-year journey through bioconversion of lignocellulosic wastes to mushrooms and dietary supplements. Int. J. Med. Mushrooms 3, 299–310. >> doi.org/10.1615/intjmedmushr.v3.i2-3.40
Chayka, O.V., Fedotov, O.V., 2014. Otsinka ekolohichnoho stanu dovkillya z vykorystannyam prooksydantno-antyoksydantnoyi aktyvnosti kul’tur bazydiomitsetiv [The ecology estimation of environment state using prooxidant-antioxidant activity of Basidiomycetes cultures]. Bioresursy i Pryrodokorystuvannya 6, 5–11 (in Ukrainian).
Droge, W., 2002. Free radicals in the physiological control of cell function. Physiol. Rev. 82, 47–95. >> doi.org/10.1152/physrev.00018.2001
Dudka, Y.A., Wasser, S.P., Éllanskaya, Y.A., 2003. Metody eksperimental’noy mikologii [Methods of experimental mycology]. Naukova Dumka, Kiev (in Russian).
Eriksson, K.E.L., Blanchette, R.A., Ander, P., 1990. Microbial and enzymatic degradation of wood and wood components. Springer-Verlag, Berlin. >> doi.org/10.1007/978-3-642-46687-8
Fedotov, O.V., Chayka, O.V., Voloshko, T.E., Velyhods’ka, A.K., 2012. Kolektsiya kul’tur shapynkovykh hrybiv – osnova mikolohichnykh doslidzhen’ ta stratehiyi zberezhennya bioriznomanittya bazydiomitsetiv [Culture Collection of mushrooms – the basis of mycological research and biodiversity conservation strategies Basidiomycetes]. Visnyk Donets’koho Universytetu 1, 209–213 (in Ukrainian).
Halliwell, B., 2006. Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol. 141, 312–322. >> doi.org/10.1104/pp.106.077073
Kapich, A.N., 2010. Oxidizability of unsaturated fatty acids and of a non-phenolic lignin structure in the manganese peroxidase-dependent lipid peroxidation system. Enzyme Microb. Technol. 46(2), 136–140. >> doi.org/10.1016/j.enzmictec.2009.09.014
Kapich, A.N., 2011. Conjugation of lipid peroxidation with degradation of lignin in wood-destroying Basidiomycetes. Microbial biotechnology: Fundamental and applied aspects: Trans. Sc. Papers. 3, 316–335.
Kapich, A.N., 2011. Sopryazheniye perekisnogo okisleniya lipidov s degradatsiyey lignina u derevorazrushayushchikh ba-zidiomitsetov [Pair of lipid peroxidation degradation of lignin in wood-basidiomycetes]. Mikrobnyye biotekhnologii: Fundamental’nyye i prikladnyye aspekty 3, 316–335 (in Rus-sian).
Kirk, P.M., Cannon, P.F., Minter, D.W., Stalpers, J.A., 2008. Dictionary of the fungi. CABI, Wallingford.
Kobzeva, T.V., Melnikov, A.R., Karogodina, T.Y., 2014. Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation. Luminescence 29, 703–710. >> doi.org/10.1002/bio.2656
Leonowicz, A., Matuszewska, A., Luterek, J., 1999. Biodegradation of lignin by white rot fungi. Fungal Genet. Biol. 27, 175–185. >> doi.org/10.1006/fgbi.1999.1150
Pham-Huy, L.A., He, H., Pham-Huyc, C., 2008. Free radicals, antioxidants in disease and health. International Journal of Biomedical Science 4, 89–96.
Pirog, T.P., Ignatova, O.A., 2009. Zahal’na biotekhnolohiya [General biotechnology]. NUHT, Kyiv (in Ukrainian).
Pozdnyakova, N.N., Nikiforova, S.V., Turkovskaya, O.V., 2010. Influence of PAHs on ligninolytic enzymes of the fungus Pleurotus ostreatus D1. Cent. Eur. J. Biol. 5(1), 83–94. >> doi.org/10.2478/s11535-009-0075-4
Prisedskiy, Y.G., 1999. Statystychna obrobka rezul’tativ biolohichnykh eksperymentiv [Statistical processing of biological experiments results]. Kassіopeya, Donetsk (in Ukrainian).
Stalnaya, I.D., Garishvili, T.G., 1977. Metod opredeleniya malonovogo dial’degida s pomoshch’yu tiobarbiturovoy kisloty [Method for determination of malondialdehyde using thiobarbituric acid] Sovremennyye Metody v Biokhimii 1, 66–68 (in Russian).
Vladimirov, Y.A., Archakov, A.I., 1972. Perekisnoye okisleniye lipidov v biologicheskikh membranakh [Lipid peroxidation in biological membranes]. Nauka, Moscow (in Russian).
Voloshko, T.E., Fedotov, O.V., 2011. Skryninh shtamiv bazydiomitsetiv za aktyvnistyu antyoksydantnykh oksydoreduktaz [Screening of basidiomycetes strains on the antioxidant activity of oxidoreductases]. Microbiology and Biotechnology 16, 69–81 (in Ukrainian).
Wasser, S.P., 2011. Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. Appl. Microbiol. Biotechnol. 89, 1323–1332. >> doi.org/10.1007/s00253-010-3067-4
Wasser, S.P., Sytnik, M., Buchalo, A.S., Solomko, E.F., 2002. Medicinal mushrooms: Past, present and future. Ukr. Bot. J. 59(5), 499–524.
Winquist, E., Moilanen, U., Mettala, A., 2008. Production of lignin modifying enzymes on industrial waste material by solid-state cultivation of fungi. Biochem. Eng. J. 42, 128–132. >> doi.org/10.1016/j.bej.2008.06.006
Zhil’cova, Y.V., 2011. Zavisimost’ antioksidantno-prooksidantnogo ravnovesiya v makrofitah ot urovnya antropogennoj nagruzki [Dependence of antioxidant-prooxidant balance in macrophytes from anthropogenic stress level]. Tr. BGU 6, 47–54.