Antibacterial effect of essential oils of two plants Eucalyptus camaldulensis and Artemisia herba alba on some bacterial strains

  • H. Fenghour Larbi Tebessi University
  • H. Bouabida Larbi Tebessi University
  • D. Dris Larbi Tebessi University
  • M. Houhamdi 8 May 1945 University
Keywords: yield; antibacterial activity; inhibitory effect; secondary metabolites; sensitivity; aromatogram test.

Abstract

Essential oils are secondary plant metabolites and have many therapeutic properties. The aim of our study is to determine the antibacterial effect of the essential oils of two plants cultivated in a semi-arid region located in the Northeast of Algeria (Tebessa), Eucalyptus camaldulensis (Myrtaceae) and Artemisia herba alba (Asteraceae). The yield of essential oils of the two plants were 1.45 ± 0.026 and 1.21 ± 0.061 g/100 g of the dry matter of the aerial part respectively. The test of the antibacterial effect is based on the diffusion method on solid medium (sensitivity), this method allows us to determine the susceptibility or resistance of an organism vis-à-vis the sample studied. Our study reveals that E. camaldulensis essential oil had very strong activity on all bacterial strains tested, except on Pseudomonas aeruginosa and Enterococcus faecalis for which there was no inhibitory effect. However, A. herba alba essential oil had very strong activity on all bacterial strains tested except on Pseudomonas aeruginosa. The MIC of Artemisia essential oil ranged between 0.08 and 1.57 µL/mL, with the lowest activity for S. aureus and P. mirabilis (1.57 µL/mL) and the highest activity was observed against E. feacalis, E. coli, and K. pneumonia (0.09 µL/mL). The MIC of the second plant EO ranged between 0.08 and 0.36 µL/mL, with the lowest activity for P. mirabilis (0.36 µL/mL) and the highest one was observed against S. saprophyticus and E. coli (0.08 µL/mL). Statistical analysis shows that the two plants have the same efficacy against S. saprophyticus while E. faecalis, K. pneumoniae and P. mirabilis species are affected more by the essential oil of A. herba alba. While, E. camaldulensis has a higher efficiency than that of A. herba alba on the species: S. aureus and E. coli. Therefore, the essential oils of E. camaldulensis and A. herba alba suggests avenues for further non clinical and clinical studies.

References

Adam, K., Sivropoulou, A., Kokkini, S., Lanaras, T., & Arsenakis, M. (1998). Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. Journal of Agricultural and Food Chemistry, 46(5), 1739–1745.

Al-Jumaili, A., Kumar, A., Bazaka, K., & Jacob, M. V. (2018). Plant secondary metabolite-derived polymers: A potential approach to develop antimicrobial films. Polymers, 10(5), 515.

Álvarez-Martínez, F. J., Barrajón-Catalán, E., & Micol, V. (2020). Tackling antibiotic resistance with compounds of natural origin: A comprehensive review. Biomedicines, 8(10), 405.

Alzoreky, N. S., & Nakahara, K. (2003). Antimicrobial activity of extracts from some edible plants commonly consumed in Asia. International Journal of Food Microbiology, 80(3), 223–230.

Asdadi, A., Hamadouch, A., Gharby, S., & Idrissi Hassani, L. M. (2020). Chemical characterization of essential oil of Artemisia herba-alba Asso and his possible potential against COVID-19. Journal of Analytical Sciences and Applied Biothechnology, 2(2), 67–72.

Ashraf, M., Ali, O., Anwar, F., & Hussain, A. (2010). Composition of leaf essential oil of Eucalyptus camaldulensis. Asian Journal of Chemistry, 22(3), 1779–1786.

Bagci, E., & Digrak, M. (2003). Antimicrobial activity of essential oils of some Abies (Fir) species from Turkey. Flavour and Fragrance Journal, 11(4), 251–256.

Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79.

Baptista-Silva, S., Borges, S., Ramos, O. L., Pintado, M., & Sarmento, B. (2020). The progress of essential oils as potential therapeutic agents: A review. Journal of Essential Oil Research, 32(4), 279–295.

Boland, D. J., Brophy, J. J., & House A. P. N. (1991). Eucalyptus leaf oils: Use, chemistry, distillation and marketing. Inkata Press, Melbourne.

Bouabida, H., & Dris, D. (2020). Effect of rue (Ruta graveolens) essential oil on mortality, development, biochemical and biomarkers of Culiseta longiareolata. South African Journal of Botany, 133, 139–143.

Bouvet, J. (2013). Les Eucalyptus dans le monde, L’eucalyptus une essence majeure pour le reboisement à Madagascar, Antananarivo-Madagascar, Ankatso. Université d’Antananarivo.

Cermelli, C., Fabio, A., Fabio, G., & Quaglio, P. (2008). Effect of Eucalyptus oil on respiratory bacteria and viruses. Current Microbiology, 56(1), 89–92.

Chalchat, J. C., Gary, R. P., Sidibe, L., & Harama, M. (2000). Aromatic plants of Mali (V): Chemical composition of four Eucalyptus species implanted in Mali, Eucalyptus camaldulensis, E. torelliana, E. citriodora, E. tereticornis. Journal of Essential Oil Research, 12(6), 695–701.

Chao, S. C., Young, D. G., & Oberg, C. J. (2000). Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of Essential Oil Research, 12(5), 639–649.

Chao, S. C., Young, D. G., Oberg, C. J., & Nakaoka, K. (2008). Inhibition of methicillin-resistant Staphylococcus aureus (MRSA) by essential oils. Flavour and Fragrance Journal, 23(6), 444–449.

Chaudhuri, A., Aqil, A., & Qadir, A. (2020). Herbal cosmeceuticals: New opportunities in cosmetology. Trends in Phytochemical ResearchTrend, 4(3), 117–142.

Cimanga, K., Kambu, K., Tona, L., Apers, S., De Bruyne, T., Hermans, N., Totté, J., Pieters, L., & lietinck, A. J. (2002). Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. Journal of Ethnopharmacology, 79(2), 213–220.

Daroui-Mokaddem, H. (2012). Etude phytochimique et biologique des especes Eucalyptus globulus (Myrtaceae), Smyrnium olusatrum (Apiaceae), Asteriscus maritimus et Chrysanthemum trifurcatum (Asterarceae). Thèse de Doctorat en Biochimie Appliquée. Universite Badji Mokhtar-Annaba.

De Billerbeck, V. G., Roques, C., Vaniere, P., & Marquier, P. (2002). Activité antibactérienne et antifongique de produits à base d'huiles essentielles. Hygiennes, 3, 248–251.

Delaquis, P. J., Stanich, K., Girard, B., & Mazza, G. (2002). Antimicrobial activity of individual and mixed fractions of dill, cilanto, coriander and Eucalyptus essential oils. International Journal of Food Microbiology, 74, 101–109.

Dias Barzon, C., de Medeiros, F., Moraes, R. E., Monteiro da Silva, L. C., Massambani, C., Takemura, O. S., & Gazim, Z. C. (2008). Preliminary study of homeopathic treatment of subclinical mastitis evaluated through somatic cells count and California mastitis test. International Journal of High Dilution Research, 7(24), 147–151.

Dob, T., & Benabdelkader, T. (2006). Chemical composition of the essential oil of Artemisia herba-alba Asso grown in Algeria. Journal of Essential Oil Research, 18(6), 685–690.

Donay, J. L., Fernandes, P., Lagrange, P. H., & Herrmann, J. L. (2007). Evaluation of the inoculation procedure using a 0.25 McFarland standard for the BD phoenix automated microbiology system. Journal of Clinical Microbiology, 45(12), 4088–4089.

Esquenazi, D. M. D., Wigg, M. M., Miranda, H. M., Rodrigues, J. B., Tostes, S., Rozental da Silva, A. J., & Alviano, C. S. (2002). Antimicrobial and antiviral activities of polyphenolics from Cocos nucifera Linn. (Palmae) husk fiber extract. Research in Microbiology, 153(10), 647–652.

Euzéby, J. P. (1998). Évaluation in vitro de la sensibilité des bactéries aux antibiotiques. Dictionnaire de Bactériologie Vétérinaire.

Farah, A., Satrani, B., Fechtal, M., Chaouch, A., & Talbi, M. (2001). Composition chimique et activités antibactérienne et antifongique des huilesessentielles extraites des feuilles d' Eucalyptus camaldulensis et de son hybride naturel (clone 583). Acta Botanica Gallica, 148(3), 183–190.

Franchomme, P. (1999). L'aromathérapie thérapeutique de pointe en médecine naturelle. Sinceiro Entreprises Ltd., Hong Kong.

Ghasemian, A., Eslami, M., Hasanvand, F., Bozorgi, H., & Al-Abodi Hiba, R. (2019). Eucalyptus camaldulensis properties for use in the eradication of infections. Comparative Immunology, Microbiology and Infectious Diseases, 65, 234–237.

Ghulam, M., Rawaba, A., Asia, A., Sumaira, S., & Amer, J. (2017). Bioactive compounds from medicinal plants and their importance in drug discovery in Pakistan. Matrix Science Pharma, 1(1), 17–26.

Güllüce, M., Sökmen, M., Daferera, D., Aǧar, G., Özkan, H., Kartal, N., Polissiou, M., Sokemen, A., & Şahi̇n, F. (2003). In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. Journal of Agricultural and Food Chemistry, 51(14), 3958–3965.

Habeeb, F., Shakir, F., Bradbury, F., Cameron, P., Taravati, M. R., Drummond, A. J., Gray, A. I., & Ferro, V. A. (2007). Screening methods used to determine the antimicrobial properties of Aloe vera inner gel. Methods, 42(4), 315–320.

Harauma, A., Murayama, T., Ikeyama, K., Sano, H., Arai, H., Takano, R., Kita, T., Hara, S., Kamei, K., & Yokode, M. (2007). Mulberry leaf powder prevents atherosclerosis in apolipoprotein E-deficient mice. Biochemical and Bio-physical Research Communications, 358(3), 751–756.

Hudaib, M. M., & Aburjai, T. A. (2006). Composition of the essential oil from Artemisia herba-alba grown in Jordan. Journal of Essential Oil Research, 18(3), 301–304.

Inouye, S., Takizawa, T., & Yamaguchi, H. (2001). Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. Journal of Antimicrobial Chemotherapy, 47(5), 565–573.

Juteau, F., Msotti, V., Bessiene, J. M., & Viano, J. (2002). Compositional charateristics of essential oils of Artemisia vulgris L. (Mugwort) from North Lithuani. Biochemical Systematics and Ecology, 17, 12–15.

Kumar, A. (1988). Antibacterial properties of some Eucalyptus oils. Fitoterapia, 59, 113–123.

Kumar, M., Devi, A., Sharma, M., Kaur, P., & Mandal, U. K. (2020). Review on perfume and present status of its associated allergens. Journal of Cosmetic Dermatology, 30, 1065–1070.

Makhlouf, L., Bey, Z., & Khodir, M. (2016). Essential oils composition, antibacterial and antioxidant activities of hydrodistillated extract of Eucalyptus globulus fruits. Journal of Microbial and Biochemical Technology, 89, 167–175.

Mazari, K., Bendinerad, N., & Benkhechi, C. (2010). Chemical composition and antimicrobial activity of essential oil isolated from Algerian Juniperus phoenicea L. and Cupressus sempervirens L. Médicinal Plant Research, 4(10), 959–964.

Mehani, M. (2015). Activité antimicrobienne des huiles essentielles d’Eucalyptus camaldulensis dans la région d’Ouargla. Thèse de doctorat, Microbiologie. Ouargla. Faculté des Sciences de la Nature et de la Vie, Département des Sciences Biologiques.

Munita, J. M., & Arias, C. A. (2016). Mechanisms of antibiotic resistance. In: Kudva, I. T., Cornick, N. A., Plummer, P. J., Zhang, Q., Nicholson, T. L., Bannantine, J. P., & Bellaire, B. H. (Eds.). Virulence mechanisms of bacterial pathogens. 5th edition. American Society for Microbiology. Pp. 481–511.

Murray, P. R., Baron, E. J., Jorgenson, J. H., Pfaller, M. A., & Yolken, R. H. (2003). Manual of clinical microbiology. 8th edition. American Society for Microbiology Press, Washington.

Nair, R., Vaghasiya, Y., & Chanda, S. (2008). Antibacterial activity of Eucalpytus citriodora Hk. oil on few clinically important bacteria. African Journal of Biotechnology, 7, 25–26.

Nedjai, S. (2017). Activité antimicrobienne des huiles essentielles. Mémoire de mater: Ecologie microbienne. Université A. MIRA Bejaia, Bejaia.

Ouraïni, D., Agoumi, A., Ismaïli-Alaoui, M., Alaoui, K., Cherrah, Y., Alaoui, M. A., & Belabbas, M. A. (2007). Activité antifongique de l’acide oléique et des huiles essentielles de Thymus saturejoides L. et de Mentha pulegium L., comparée aux antifongiques dans les dermatoses mycosiques. Phytothérapie, 5(1), 6–14.

Pérez-Nicolas, M., Vibrans, H., Romero-Manzanares, A., Saynes-Vasquez, A., Luna-Cavazosa, M., Flores-Cruz, M., & Lira-Saade, R. (2017). Patterns of knowledge and use of medicinal plants in Santiago Camotlán, Oaxaca, Mexico. Economic Botany, 71(3), 209–223.

Pibiri, M. C. (2005). Assainissement microbiologique de l'air et des systèmes de ventilation au moyen d'huiles essentielles. Thèse de doctorat des sciences. École Polytechnique Fédérale de Lausanne.

Ponce, A. G., Fritz, R., del Valle, C., & Roura, S. I. (2003). Antimicrobial activity of essential oils on the native microflora of organic Swiss chard. LWT – Food Science and Technology, 36(7), 679–684.

Rabanal, R. M., Arias, A., Prado, B., Hernández-Pérez, M., & Sánchez-Mateo, C. C. (2002). Antimicrobial studies on three species of Hypericum from the Canary Islands. Journal of Ethnopharmacology, 81, 287–292.

Rios, J. L., & Recio, M. C. (2005). Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology, 100, 80–84.

Rota, C., Carramiñan, J. J., Burillo, J., & Herrera, A. (2004). In vitro antimicrobial activity of essential oils from aromatic plants against selected foodborne. Pathogens Journal of Food Protection, 67(6), 1252–1256.

Seyyednejad, S. M., Koochak, H., Darabpour, E., & Motamedi, H. (2010). A survey on Hibiscus rosa sinensis L., Alcea rosea L. and Malva neglecta Wallr. as antibacterial agents. Asian Pacific Journal of Tropical Medicine, 3(5), 351–355.

Shunying, Z., Yang, Y., Huaidong, Y., Yue, Y., & Guolin, Z. (2005). Chemical composition and antimicrobial activity of the essential oils of Chrysanthemum indicum. Journal of Ethnopharmacology, 96, 151–158.

Silva, J. A. A., Vizotto, V. J., Giorgi, E., Macedo, S. G., & Marques, L. F. (1994). Plantas medicinais, caracterização e cultivo. Epagri. Boletim Tecnico. Empresa de Pesquisa Agropecuaria e Difusao de Tecnologia de Santa Catarina S.A., Florianopolis (Brazil), 68, 1–71.

Smith-Palmer, A. (1998). Antimicrobial properties of plant essentialoils and essences against five important food-borne pathogens. Letters in Applied Microbiology, 26(2), 118–122.

Tassou, C. C., Koutsoumanis, K., & Nychas, G. J. E. (2000). Inhibition of Salmonella enteridis and Staphyloccus aureus on nutrient broth by mint essential oil. Food Research International, 48, 273–280.

Tesche, S., Metternich, F., Sonnemann, U., Engelke, J.-C., & Dethlefsen, U. (2008). The value of herbal medicines in the treatment of acute non-purulent rhinosinusitis. European Archives of Oto-Rhino-Laryngology, 265(11), 1355–1359.

Tohidi, B., Rahimmalek, M., & Trindade, H. (2019). Review on essential oil, extracts composition, molecular and phytochemical properties of Thymus species in Iran. Industrial Crops and Products, 134, 89–99.

Traore, N., Sidibe, L., Bouare, S., Harama, D., Somboro, A., Fofana, B., Diallo, D., Figueredo, G., & Chalchat, J. C. (2013). Activités antimicrobiennes des huiles essentielles de Eucalyptus citriodora Hook et Eucalyptus houseana W. Fitzg. ex Maiden. International Journal of Biological and Chemical Sciences, 7(2), 800–804.

Trivedi, N. A., & Hotchandani, S. C. (2004). A study of the antimicrobial activity of oil of Eucalyptus. Indian Journal of Pharmacology, 36, 93–94.

Trombetta, D., Castelli, F., Sarpietro, M. G., Venuti, V., Cristani, M., Daniele, C., Saija, A., Mazzanti, G., & Bisignano G. (2005). Mechanisms of antibacterial action of three monoterpenes. Antimicrobiol Agents and Chemotherapy, 49(6), 2474–2478.

Yang, X.-W., Li, S.-M., Shen, Y.-H., & Zhang, W.-D. (2008). Phytochemical and biological studies of Abies species. Chemistry and Biodiversity, 5(1), 56–81.

Younessi-Hamzekhanlu, M., Sanjari, S., Dejahang, A., Karkaj, E. S., Nojadeh, M. S., Gönenç, T. M., & Ozturk, M. (2020). Evaluation of essential oil from different Artemisia fragrans Willd. Populations: Chemical composition, antioxidant, and antibacterial activity. Journal of Essential Oil Bearing Plants, 23(6), 1218–1236.

Yu, J. Q., Lei, J. C., Yu, H., Cai, X., & Zou, G. L. (2004). Chemical composition and antimicrobial activity of the essential oil of Scutellaria barbata. Phytochemistry, 65(7), 881–884.

Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., & Brygadyrenko, V. V. (2019). Antimicrobial activity of 50 plant extracts. Biosystems Diversity, 27(2), 163–169.

Zazharskyi, V. V., Davydenko, P. О., Kulishenko, O. М., Borovik, I. V., & Brygadyrenko, V. V. (2020). Antibacterial and fungicidal activities of ethanol extracts from Cotinus coggygria, Rhus typhina, R. trilobata, Toxicodendron orientale, Hedera helix, Aralia elata, Leptopus chinensis and Mahonia aquifolium. Regulatory Mechanisms in Biosystems, 11(2), 305–309.

Zazharskyi, V., Davydenko, P., Kulishenko, O., Borovik, I., Brygadyrenko, V., & Zazharska, N. (2019). Antibacterial activity of herbal infusions against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa in vitro. Magyar Állatorvosok Lapja, 141, 693–704.

Published
2021-04-15
Section
Articles