Species-specific morphological characteristics of adult and embryonic Capillariа obsignata roundworms (Nematoda, Capillariidae)


  • V. A. Yevstafyeva Poltava State Agrarian Academy
  • V. V. Stybel Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj
  • T. A. Sharavara Poltava State Agrarian Academy
  • V. V. Melnychuk Poltava State Agrarian Academy
  • I. О. Yasnolob Poltava State Agrarian Academy
  • А. А. Antipov Bila Tserkva National Agrarian University
Keywords: chicken, abundance index, adult roundworm, nematode eggs, differential characters

Abstract

The abundance of Capillaria obsignata Madsen, 1945, the causative agent of chicken capillariasis, was studied in the central region of Ukraine. Differential morphometric characters are determined for adult males and females of C. obsignata. The species-specific variability of its morphological parameters is characterized. New data is received on the meristic characters of C. obsignata eggs extracted from different substrates. The stages of embryonic development are determined in laboratory cultures of these nematodes, taking into account their meristic and morphological changes. Capillaria obsignata parasites are very common at the poultry farms of Poltava and Kyiv regions of Ukraine. Their abundance index is 3.4, and the intensity of infection varies from 3 to 317 specimens. Differential morphometric species-specific characters of adult C. obsignata males are the shape and size of the pseudobursa, spicule, spicule sheath. Adult females of the species can be identified by the shape and size of the vulva, the distance from the esophagus end to the vulva, and the length of the vagina. The meristic characters of nematode eggs, though typical for the species, may change depending on the sampled substrate. The parasite’s embryonic development involves five morphologically and meristically different stages. The invasive larvae of this species developed in the eggs before the 12th day of culturing in laboratory conditions at 25 °С. The invasive larvae’s viability was 90.3 ± 1.5%.

References

D’ávila, S., Bessa, E. C. A., & Rodrigues, M. L .A. (2017). Estudo morfométrico e aspectos taxonômicos de Baruscapillaria obsignata (Nematoda, Capillariidae), parasito de Columba livia (Aves, Columbidae). Revista Brasileira de Zoociências, 18(2), 35–44.

D’ávila, S., Bessa, E. C. A., Souza-Lima, S., & Rodrigues, M. L. A. (2012). Biased sex ratio and niche restriction in Baruscapillaria obsignata (Madsen 1945) (Nematoda, Capillariidae) from Columba livia (Aves, Columbidae). Journal of Helminthology, 86(4), 401–405.

Gagarin, V. G. (1971). Znachenie vidovyh i rodovyh kriteriev na primere kapilljariid [The significance of specific and generic criteria on the example of Capillariidae]. Helminthological studies in Kyrgyzstan. Ilim, Frunze (in Russian).

Gürler, А. Т., Bölükbaş, C. S., Pekmezci, G. Z., Umur, Ş., & Açıcı, М. (2012). Samsun’da Sülünlerde (Phasianus colchicus) Nekropsi ve Dışkı Bakısında Saptanan Helmintler. Türkiye Parazitoloji Dergisi, 36, 222–722.

Hassouni, T., & Belghyti, D. (2006). Distribution of gastrointestinal helminths in chicken farms in the Gharb region – Morocco. Parasitology Research, 99(2), 181–183.

Ivashkin, V. M., Kontrimavichus, V. L., & Nazarova, N. S. (1971). Metody sbora i izuchenija gel’mintov nazemnyh pozvonochnyh [Methods for collection and study of terrestrial mammals’ helminthes.]. Nauka, Moscow (in Russian).

Kajerová, V., & Baruš, V. (2005). Psittacine birds (Aves: Psittaciformes) as new hosts of Baruscapillaria obsignata (Nematoda: Capillariidae). Acta Veterinaria Brno, 74, 571–574.

Kaufmann, F., Daş, G., Sohnrey, В., & Gauly, М. (2011). Helminth infections in laying hens kept in organic free range systems in Germany. Livestock Science, 141(2–3), 182–187.

Lapach, S. N., Chubenko, A. V., & Babich, P. N. (2001). Statisticheskie metody v mediko-biologicheskih issledovanijah s ispol’zovaniem Excel [Statistical methods in medical and biological research using Excel]. Morion, Kyiv (in Russian).

Levine, P. P. (1938). Infection of the chicken with Capillaria columbae (Rud.). Journal of Parasitology, 24(1), 45–52.

Lomakin, V. V., & Romashov, B. V. (1987). Morfolgo-taksonomicheskij analiz i filogeneticheskie otnoshenija nematod semejstva Capillariidae Railliet, 1915 [Morphological-taxonomic analysis and phylogenetic relationships of nematodes of the family Capillariidae Railliet, 1915]. Morphology, Taxonomy and Ecology of Helminthes of Plants and Animals, 35, 87–95 (in Russian).

Moravec, F., & Muzzall, P. M. (2009). New species of freitas Capillaria (Nematoda: Capillariidae) from the intestine of Cottus caeruleomentum (Teleostei: Cottidae) in Maryland. Journal of Parasitology, 95(4), 987–990.

Moravec, F., Scholz, T., & Našincová, V. (1994). The systematic status of Trichosoma carbonis Rudolphi, 1819 and a description of Baruscapillaria rudolphii n. sp. (Nematoda: Capillariidae), an intestinal parasite of cormorants. Systematic Parasitology, 28(2), 153–158.

Mukaratirwa, S., & Khumalo, М. Р. (2010). Prevalence of helminth parasites in free-range chickens from selected rural communities in KwaZulu-Natal province of South Africa. Journal of the South African Veterinary Association, 81(2), 97–101.

Mukaratirwa, S., Hove, T., Esmann, J. B., Hoj, C. J., Permin, A., & Nansen, P. (2001). A survey of parasitic nematode infections of chickens in rural Zimbabwe. Onderstepoort Journal of Veterinary Research, 68(3), 183–186.

Nasirov, A. M. (1981). Tonkoe stroenie polovoj sistemy samok Capillaria obsignata Madsen, 1945 [The fine structure of female reproductive system of Capillaria obsignata Madsen, 1945]. Proceedings of the National Academy of Sciences of Azerbaijan, 5, 76–77 (in Russian).

Paramonov, A. A. (1957). O principah taksonomicheskoj differencirovki v nematologii [On the principles of taxonomic differentiation in nematology]. Russian Journal of Zoology, 34(5), 641–653 (in Russian).

Park, S.-I., & Shin, S. S. (2010). Concurrent Capillaria and Heterakis infections in zoo rock partridges, Alectoris graeca. Korean Journal of Parasitology, 48(3), 253–257.

Pasechnik, V. E. (2013). Kapilljariidy ptic i ih jepizooticheskoe znachenie v Moskovskom regione [Bird capillariases and their epizootic importance in the Moscow region]. Theory and Practice of the Struggle Against Parasitic Diseases, 14, 298–300 (in Russian).

Petrochenko, V. I., & Kotelnikov, G. A. (1976). Gel’mintozy ptic [Helminthic diseases of birds]. Kolos, Moscow (in Russian).

Ripolovskyi, O. I., & Yuskiv, I. D. (2010). Dyplostomozy koropa v nahulnykh stavakh Pivnichno-Zakhidnoho Peredkarpattia [Diplostomoses of carp in the ponds of the North-Western Prykarpathia]. Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, 12(3), 197–205 (in Ukrainian).

Romashov, B. V. (1985). Morfologicheskie osobennosti skorlupy jaic kapilljariid (Nematoda, Capillariidae) [The morphological specifics of eggshell in Capillariidae (Nematoda, Capillariidae)]. Parasitology, 19(5), 399–402 (in Russian).

Schou, T. W., Permin, A., Juul-Madsen, H. R., & Sǿrensen, P. (2007). Gastrointestinal helminths in indigenous and exotic chickens in Vietnam: Association of the intensity of infection with the Major Histocompatibility Complex. Parasitology, 134, 561–573.

Skrjabin, K. I. (1928). Metod polnyh gel’mintologicheskih vskrytij pozvonochnyh, vkljuchaja cheloveka [The method of complete helminthological autopsy of vertebrates, including humans]. First Moscow State University, Moscow (in Russian).

Skrjabin, K. I., Shikhobalova, N. P., & Orlov, I. V. (1957). Trihocefalidy i kapilljariidy zhivotnyh i cheloveka i vyzyvaemye imi zabolevanija [Trichocephalids and capillariids of animals and man and the diseases caused by them. The essentials of nematodology]. Academy of Sciences of the USSR, Moscow (in Russian).

Tamaru, M., Yamaki, S., Jimenez, L. A., & Sato, H. (2015). Morphological and molecular genetic characterization of three Capillaria spp. (Capillaria anatis, Capillaria pudendotecta, and Capillaria madseni) and Baruscapillaria obsignata (Nematoda: Trichuridae: Capillariinae) in avians. Parasitology Research, 114(11), 4011–4022.

Tanveer, S., Ahad, S., & Chishti, M. Z. (2015). Morphological characterization of nematodes of the genera Capillaria, Acuaria, Amidostomum, Streptocara, Heterakis, and Ascaridia isolated from intestine and gizzard of domestic birds from different regions of the temperate Kashmir valley. Journal of Parasitic Diseases, 39(4), 745–760.

Tiersch, K. M., Daş, G., Samson-Himmelstjerna, G. V., & Gauly, M. (2013). The role of culture media on embryonation and subsequent infectivity of Capillaria obsignata eggs. Parasitology Research, 112(1), 357–364.

Timi, J. T., Rossin, M. A., & Lanfranchi, А. L. (2006). A new species of Capillaria (Nematoda: Capillariidae) parasitizing conger Orbignianus (Pisces: Congridae) from Argentina. Journal of Parasitology, 92(3), 628–630.

Wakelin, D. (1965). Experimental studies on the biology of Capillaria obsignata Madsen, 1945, a nematode parasite of the domestic fowl. Journal of Helminthology, 39(4), 399–412.

Wakelin, D. (1966). The genus Capillaria Zeder, 1800 (Nematoda) in British passerine birds. Parasitology, 56(1), 161–170.

Wehr, E. E. (1939). Studies on the development of the pigeon capillarid, Capillaria columbae. United States Department of Agriculture, Washington.

Yazwinski, Т., Tucker, C., Wray, E., Jones, L., Johnson, Z., Steinlage, S., & Bridges, J. (2013). A survey on the incidence and magnitude of intestinal helminthiasis in broiler breeders originating from the southeastern United States. Journal of Applied Poultry Research, 22(4), 942–947.
Published
2018-01-23
Section
Articles

Most read articles by the same author(s)