The current state of the population of the golden potato cyst nematode Globodera rostochiensis (Nematoda, Heteroderidae) in the northwest of Ukraine


Keywords: numbers dynamics; reproduction coefficient; infection rate

Abstract

The golden potato cyst nematode (GPCN) Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 is a highly specific parasite of the roots of the nightshade plants (Solanaceae). Thus, the state of the pest population demands constant monitoring and control of distribution and numbers. The distribution of G. rostochiensis in Volyn region of Ukraine was studied in 2008–2018 using the data of the state institution the Volyn Regional Phytosanitary Laboratory. The present article gives the analysis of the study results. The disease foci were detected by visual above-ground inspection of potato plantings, also by manually collecting soil samples before planting the potatoes and after harvesting, and consequently analyzing theme. The initial (pre-planting) and conclusive (after harvest) population density of GPCN in the soil was determined by the number of cysts and the mean number of larvae and eggs in cysts obtained from 100 cm3 of soil. Cysts were isolated from soil samples by the standard funnel flotation method. The dead and living larvae were identified visually by the shape of the body and the state of internal organs. According to the results of assessing pre-planting and post-harvest nematode numbers in soil, the reproduction coefficient Рf/Рі was calculated. In Volyn region, G. rostochiensis was first observed in 1968 on farmland and since then, the parasite has spread. Pest foci were recorded in 15 districts of the region in 303 settlements on the area of 946.123 hectares. It was however found that during the latest decade, the area of soils affected by G. rostochiensis in Volyn region decreased by 147.647 hectares. The largest infected areas (over 100 ha) were located in Kovel, Rozhyshche and Manevistky districts, the least infected area was observed in Ivanychi district of the region. The pest was not found in Lutsk district. The highest infection rate was recorded in Rozhyshche district. The highest ratio of viable cysts was observed in the soils of Volodymyr-Volynsky and Rozhyshche districts. The soils of the southern districts (located in the natural zone of forests and steppe) of the region demonstrated 1.5 times higher infection rates compared to soils of the northern districts (in the natural zone of mixed forests). The soils of the southern districts also harboured stable and strong pest populations. The pre-planting soil infection rates proved to directly depend on the reproduction coefficient of GPCN. If the values of Рі, initial infection rate, were lower than 1,000 larvae and eggs per 100 cm3, the reproduction coefficient was 1.18. Increase in the pre-planting infection rate to 2,000 eggs and larvae per 100 cm3 did not affect the reproduction coefficient. At approximately 5,000 eggs and larvae per 100 cm3 the reproduction coefficient exceeded 2, which should be considered in developing the pest control measures.

References

Babych, A. G., Babych, A. A., Suhareva, R. D., & Statkevich, A. A. (2015). Vlijanie sevooborota na chislennost zolotistoj kartofelnoj nematody [The effect of crop rotation on the number of golden potato nematodes]. Agronom, 4, 134–136 (in Ukrainian).
Baker, R., Candresse, T., Dormannsné Simon, E., Gilioli, G., Grégoire, J. C., Jeger, M. J., Evtimova Karadjova, O., Lövei, G., Makowski, D., Manceau, C., Navajas, M., Puglia, A. P., Rafoss, T., Rossi, V., Schans, J., Schrader, G., Urek, G., van Lenteren, J. C., Vloutoglou, I., Winter, S., & Zlotina, M. (2012). Scientific opinion on the risks to plant health posed by European versus non-European populations of the potato cyst nematodes Globodera pallida and Globodera rostochiensis. EFSA Journal, 10(4), 1–71.
Brodie, B. B., & Mai, W. F. (1989). Control of the golden nematode in the United States. Annual Review of Phytopathology, 27, 443–461.
Hockland, S., Niere, B., Grenier, E., Blok, V., Phillips, M., Den Nijs, L., Anthoine, G., Pickup, J., & Viaene, N. (2012). An evaluation of the implications of virulence in non-European populations of Globodera pallida and G. rostochiensis for potato cultivation in Europe. Nematology, 14, 1–13.
Korzhuk, R. D., Melnyk, P. O., Pruncev, S. Y., Ostryk, I. M., & Babych, A. H. (2005). Metodychni rekomendaciyi z vyyavlennya kartoplyanyx cystoutvoryuyuchyx nematod [Methodical recommendations for the detection of potato cystogenic nematodes]. Zelena Bukovyna, Chernivci (in Ukrainian).
Mimee, B., Dauphinais, N., & Belair, G. (2015). Life cycle of the golden cyst nematode, Globodera rostochiensis, in Quebec. Canada Journal of Nematology, 47(4), 290–295.
Minnis, S. T., Haydock, P. P. J., Ibrahim, S. K., Grove, I. G., Evans, K., & Russell, M. D. (2002). Potato cyst nematodes in England and Wales – occurrence and distribution. Annals of Applied Biology, 140, 187–195.
Oka, Y. (2010). Mechanisms of nematode suppression by organic soil amendments. Applied Soil Ecology, 44, 101–115.
Osypchuk, A. A., Taktaev, B. A., Sigareva, D. D., & Pylypenko, L. A. (2018). Breeding for resistance to the potato cyst nematode in Ukraine. Czech Journal of Genetics and Plant Breeding, 38(3–4), 158–159.
Philis, J. (1980). Life history of the potato cyst-nematode Globodera rostochiensis in Cyprus. Nematologica, 26, 295–301.
Polozhenets, V. M., Hurmanchuk, O. V., & Halahan, T. O. (2010). Biolohichni ta morfolohichni osoblyvosti rozvytku zolotystoyi cystoutvoryuyuchoyi kartoplyanoyi nematody u zoni Polissya Ukrayiny [Biological and morphological peculiarities of golden cyst-forming potato nematode development in the Polissya area of Ukraine]. Naukovi Dopovidi Nacionalnoho Universytetu Bioresursiv i Pryrodokorystuvannya, 6, 28 (in Ukrainian).
Przetakiewicz, A. (2013). The first report of Globodera rostochiensis pathotypes Ro5 occurrence in Poland. Plant Disease, 97(8), 1125.
Pylypenko, L. A. (1998). Poshyrennya Globodera rostochiensis (Tylenchida, Heteroderidae) v Ukrayini [Distribution of Globodera rostochiensis (Tylenchida, Heteroderidae) in Ukraine]. Vestnik Zoologii, 32(5–6), 139–142 (in Ukrainian).
Renčo, M. (2007). Comparison of the life cycle of potato cyst nematode (Globodera rostochiensis) pathotype Ro1 on selected potato cultivars. Biologia, 62(2), 195–200.
Scholte, K. (2000). Effect of potato used as trap crop on potato cyst nematodes and other soil pathogens and on the growth of a subsequent main potato crop. Annals of Applied Biology, 136, 229–238.
Seinhorst, J. W. (1984). Relation between population density of potato cyst nematode and measured degrees of susceptibility (resistance) of resistant potato cultivars and between this density and cyst content in the new generation. Nematologica, 30, 66–76.
Suhareva, R. D., & Babych, A. H. (2013). Tryvalist rozvytku ta dynamika chyselnosti zolotystoyi kartoplyanoyi cystoutvoryuyuchoyi nematody v umovax Zaxidnoho Lisostepu Ukrayiny [Duration of development and dynamics of the number of golden potato cyst in the accession nematode in the conditions of the Western Forest-Steppe of Ukraine]. Zahyst i Karantyn Roslyn, 59, 285–293 (in Ukrainian).
Sіgareva, D. D., & Pylypenko, L. A. (1998) Control methods for potato nematodes in Ukraine 1. Bulletin OEPP/EPPO, 28(4), 529–532.
Sіgareva, D. D., Halahan, T. O., & Nikishycheva, K. S. (2010). Novyj pidhid do monitorinhu nematodoziv roslyn [New approach to the monitoring of plant nematodes]. Vestnik Zoologii, 23, 183–190 (in Ukrainian).
Sіgareva, D. D., Kozhushko, N. S., Buchyk, S. V., & Sіlchak, N. Y. (2014). Napryamy vyrishennya problemy hloboderozu kartopli v pivnichno-shidnomu rehioni Ukrayiny [Directions of solving the problem of potato globoderosis in the north-eastern region of Ukraine]. Visnyk Sumskoho Nacionalnoho Ahrarnoho Universytetu, Seriya Ahronomiya i biolohiya, 27, 233–237 (in Ukrainian).
van den Elsen, S., Ave, M., Schoenmakers, N., Landeweert, R., Bakker, J., & Helder, J. (2012). A rapid, sensitive and cost-efficient assay to estimate viability of potato cyst nematodes. Phytopathology, 102, 140–146.
Webley, D. P., & Jones, F. G. W. (1981). Observations on Globodera pallida and G. rostochiensis on early potatoes. Plant Pathology, 30, 217–224.
Yu, Q., Ye, W., Sun, F., & Miller, S. (2010). Characterization of Globodera rostochiensis (Tylenchida: Heteroderidae) associated with potato in Quebec, Canada. Canadian Journal of Plant Pathology, 32(2), 264–271.
Published
2019-03-02
Section
Articles