New approaches to quality control for cultures of insects for rearing


  • T. Y. Markina H.S. Skovoroda Kharkiv National Pedagogical University
Keywords: insect cultivation, quality control, taxis, viability

Abstract

An analytical review of existing methods of quality control for cultures of insects for rearing is presented. It is shown that there is a need to search for new approaches to solving this problem. The suggested methods for quality control for insect cultures are based on the rule of taxis intensity dependence on insect viability level. Testing of new methods of control and prediction of biological material viability was carried out on several breeds of silkworm (Bombyx mori L.) and a laboratory culture of the gypsy moth (Lymantria dispar L.). It was established that the caterpillars of silkworm breeds with high viability show higher chemotaxis intensity. It was found that intensity of taxis manifestation can be considered as a criterion of population condition. Selection of the most promising breeds of silkworm can be carried out at the moment of hatching without expensive rearing. It was shown that testing of neonate caterpillars by chemotaxis intensity provides the control of viability in all stages of insect development. Evaluation of chemotaxis intensity of adult males on female sex pheromone gives the possibility to select the specimens with the highest physiological properties for hybridization as well as to predict viability for progeny. The method of biological material selection by chemotaxis intensity was improved due to decrease the time of selection from 30 to 15 minutes and preliminary starvation of these insects. Positive correlation was evaluated between general viability of silkworm culture and intensity of chemotaxis of neonate larvae to the smell of mulberry leaf during 15 minutes after 12 hours of starvation. A significant increase of viability for silkworm breeds and hybrids was registered using such selection method during the spring and summer rearing. This selection method can be used also for assessment of culture viability. A new express-method of biological material selection for insect cultures establishment has been approbated. It is based on the positive correlation between insect viability parameters and their trophotaxis intensity. It is shown that for establishment of a laboratory culture of gypsy moth it is necessary to obtain the material from populations with the highest chemotaxis intensity. It was experimentally proved that parameters of chemotaxis intensity and insect viability reflect a certain level of population heterozygosity. The higher is the intensity of chemotaxis, the higher is the relative level of population heterozygosity. This fact gives the possibility to determine the level of heterozygosity for artificial insect populations by testing larvae for chemotaxis intensity. This method may be suitable for monitoring of natural populations condition in zones with intensive anthropogenic loading. 

References

Adashkevitch, B., 1988. Standartyzatziya entomophagow [Standartization of the entomophagous]. Zaschtita Rastenij 10, 16 (in Russian).
Belyakova, N., Kozlov, E., 2010. Nowye standarty i control katchestwa pri massovom razvedenii entomofagov [New standards and quality control in mass breeding of entomophagous]. Izvestiya Sankt-Petersburgskogo Agrarnogo Universiteta 18, 104–107 (in Russian).
Benkowskaya, G., 2009. Stress reaktzii kak mekhanizm realizatzii adaptivnogo potentziala osobej i populyatzij nasekomikh [Stress reaction as a mechanism for the implementation of the adaptive capacity of individuals and populations of insects]. Novosibirsk (in Russian).
Benkowskaya, G., Saltykov, E., Sukhorukova, O., Nikolenko, A., 2006. Metabolicheskaya regulyatziya dvukh tipov fenoloksidaznoj aktivnosti v onthogeneze komnatnoj mukhi [The metabolic regulation of two types of activity in ontogenesis fenoloksidaznoy housefly]. Ontogenesis 37(20), 142–148 (in Russian).
Benkowskaya, G., Udalov, M., Khusnutdinova, E., 2008. Geneticheskaya osnova i fenotipicheskie proyavleniya rezistentnosti koloradskogo zhuka k fosfororganicheskim insekticidam [Genetic basis and phenotypic manifestations of resistance of the Colorado potato beetle to the organophosphorus insektycides]. Genetics 44(5), 638–644 (in Russian).
Braslavskyi, M., Golovko, V., Zlotin, A., Shchukin, A., Ostapenko, L., 2002. Selektziya tutovogo shelkopryada w Ukraine (dostizheniya, problemy i perspektivy) [Silkworm selection in Ukraine (achievements, problems and prospects)]. Original, Kharkov (in Russian).
Brygadyrenko, V., Ivanyshyn, V., 2015. Changes in the body mass of Megaphyllum kievense (Diplopoda, Julidae) and the granulometric composition of leaf litter subject to different concentrations of copper. J. Forest Sci. 61(9), 369–376.
Brygadyrenko, V.V., Nazimov, S.S., 2014. Nutrition of Opatrum sabulosum (Coleoptera, Tenebrionidae) when fed on leaves of trees, shrubs and liana plants in the conditions of a laboratory experiment. Baltic Journal of Coleopterology 14(1), 59–72.
Brygadyrenko, V.V., Nazimov, S.S., 2015. Trophic relations of Opatrum sabulosum (Coleoptera, Tenebrionidae) with leaves of cultivated and uncultivated species of herbaceous plants under laboratory conditions. ZooKeys 481, 57–68.
Brygadyrenko, V.V., Reshetniak, D.Y., 2014. Morphological variability among populations of Harpalus rufipes (Coleoptera, Carabidae): What is more important – the mean values or statistical peculiarities of distribution in the population? Folia Oecol. 41(2), 109–133.
Bush, G., Nesk, R., 1976. Ecological genetics of the Screwworm fly, Cochliomyia hominivorax (Diptera: Calliphoridae) and its bearing on the quality control of mass-reared insects. Environ. Entomol. 5(5), 821–826.
Chambers, D., Ashley, F., 1984. Putting the control in quality control in insect rearing. Advances and challenges in insect rearing USDA–ARS. Government Priting Office, DC, Washington, pp. 256–269.
Chernij, A., 2004. Biologichne obgruntuwannya zastosuwannya regulyatoriw zhyttediyal’nisti komakh dlya obmezhennya ikh chyselnosti [The biological justification of the using of insect vital activity regulators for limiting their abundance]. Kyiv (in Ukrainian).
Danshina, E., 2000. Novyi priem povysheniya zhysnesposobnosti tutovogo shelkopryada Bombyx mori L., putem okhlazhdeniya yaitz vo wremya diapausy [New methods of increasing the viability of the silkworm Bombyx mori L. by cooling of the eggs during diapauses]. The Kharkov Entomological Society Gazette 8(2), 55–157 (in Russian).
Dubko, L., 1995. Biologicheskie osnowy kultivirovaniya nekotorykh vidow wolnyanok (Lepidoptera: Orgyidae) [Biological bases of cultivation of some species of Tussock moths (Lepidoptera: Orgyidae)]. Мoscow (in Russian).
Elizarov, Y., Barybkina, M., 1974. Specifichnost’ dvigatelnoj reaktzii samtzov tutovogo shelkopryada pri razdrazhenii zapakhom polovogo attraktanta [Specificity motor response of male silkworm during stimulation of sexual attractant]. Biological Sciences 5, 20–22 (in Russian).
Galanova, O., Zlotin, A., Golovko, V., 1998. Otzenka i prognozirovanie kachestva porod i hybridov tutovogo shelkopryada [Estimation and forecasting of quality of breeds and hybrids of silkworm]. Original, Kharkov (in Russian).
Ghosh, S., Haldar, P., Mandal, D.K., 2014. Suitable food plants for mass rearing of the short-horn grasshopper Oxya hyla hyla (Orthoptera: Acrididae). Eur. J. Entomol. 111(3), 448–452.
Gilyarov, A., 1990. Populyatzionnaya ekologia [Population ecology]. MGU, Moscow (in Russian).
Golovko, V., Chepurnaya, N., Zlotin, A., 1995. Selektziya i kontrol’ kachestva kul’tur nasekomykh [The selection and quality control of insect’s cultures]. Original, Kharkov (in Russian).
Golovko, V., Zlotin, A., Kirichenko, I., 1992. Systema meropriyatij po optimizatzii tekhnologichezkikh protzessov razvedeniya tutovogo shelkopryada, profilaktike i bor’be z boleznyami [System of measures for process optimization of breeding silkworm, prevention and control of diseases]. Original, Kharkov (in Russian).
Golovko, V., Zlotin, O., Braslavskyi, M., Kirichenko, I., Pilipenko, B., Bojchuk, Y., Kazmiruk, V., 1998. Showkiwnytztwo. [Silkculture]. Original, Kharkov (in Ukrainian).
Greenberg, S., 1991. Nauchnye osnowy biotekhnologii proizvodstwa i primeneniya trikhogrammy [Scientific bases of biotechnology, production and using of Trichogramma]. All-Russian Research Institute of Plant Protection, St. Petersburg (in Russian).
Greenberg, S., Podberezskaya, L., 1980. Standart na Trichogrammu v bor’be s sowkami [Standard on the Trichogramma for control cutworms]. Trichogramma 2, Chisinau, pp. 32–35 (in Russian).
Greenberg, S., Podberezskaya, L., 1983. Poriwnyalna otzinka pokaznykiw yakosti trykhogrammy, rosmnozhuwanoi na yaitzyakh riznykh zhywiteliw [Comparative evaluation of the quality of Trichogramma, multiplying on the eggs of various hosts]. Zakhyst Roslyn 30, 39–41 (in Ukrainian).
Haiduk, K., Zlotіn, O., Markіna, T., 2003. Dyferentziatziya gusenutz’ shovkowychnogo showkopryadu pershogo wiku za reakzieyu perewagy na pewnyj kormowyj pidraznyk [Differentiation of caterpillars of the silkworm of the first age benefits by reaction to certain feeding stimulus]. Naukovo-Tehnіchny Bulletin 84, 43–46 (in Ukrainian).
Hoy, M.A., 1976. Genetic improvement of insects: Fact of fantasy. Environ. Entomol. 5(5), 833–839.
Huettel, M.D., 1976. Monitoring the quality of laboratory reared insects: A biological and behavioral perspective. Environ. Entomol. 5(5), 807–814.
Korolev, О.V., Brygadyrenko, V.V., 2014. Influence of individual variation in the trophic spectra of Pterostichus melanarius (Coleoptera, Carabidae) on the adaptation possibilities of its population. Folia Oecol. 41(1), 34–43.
Kulbachko, Y., Loza, I., Pakhomov, O., Didur, O., 2011. The zoological remediation of technogen faulted soil in the industrial region of the Ukraine Steppe zone. In: Behnassi, M. et al. (eds.), Sustainable agricultural development. Springer Science + Business Media, Dordrecht, Heidelberg, London, New York, 115–123.
Lapu, E., Shlyakhtich, V., Yazlovetskij, I., 1985. Primenenie enzimaticheskikh metodov dlya kontrolya sostoyaniya Trichogrammy w protzesse khraneniya [Application of enzymatic methods for control of Trichogramma state during storage]. II All-Union meeting by Trichogramma, Abstracts, Chisinau, 22–23 (in Russian).
Leppla, N.C., Ashley, T.R., 1989. Quality control in insect mass production: A review and model. Bull. Entomol. Soc. Am. 70, 33–44.
Lyashenko, Y., 1999. Pro mozhlywosti widbiru wykhidnogo materialu po zhyttezdatnosti showkovychnogo showkopryadu w riznykh napryamkakh ta yogo wplyw na geterogennist’ populyatzij [On the possibility of the selection of source material for silkworm viability in different directions and its impact on population heterogeneity]. Showkіwnitstvo 22, 59–63 (in Ukrainian).
Makarenko, G., 1975. Vliyanie kachestva yaitz zernovoi moli na razvedenie zlatoglazki obyknovennoi [The influence of the quality of the grain moth eggs on the breeding of lacewing]. Trudy VIZR 44, 620 (in Russian).
Mamedalieva, M., Polyakova, Y., Korochkin, L., 1990. Issledovanie povedencheskikh priznakov much Drosophila lummei, gomozigotnykh po nul’-allelyu gena esterasy [The study of behavior patterns among flies Drosophila lummie, homozygous by zero-allele of esterase gene]. Zh. Obshch. Biol. 51(4), 492–498 (in Russian).
Markina, T., Benkovskaya, G., 2015. Mekhanizmy podderzhaniya gomeostaza v laboratornykh populyatziyakh nasekomykh [Mechanisms of homeostasis maintenance in laboratory populations of insects]. Russ. J. Ecol. 46(4), 294–299 (in Russian).
Markina, T., Zlotin, A., 2010. Intensivnost’ proyavleniya taksisov i zhysnesposobnost’ nasekomykh: Obschebiologicheskie zakonomernosti [Intensity of taxes manifestations and viability of insects: General biological regularities]. The Kharkov Entomological Society Gazette 18(2), 66–71 (in Russian).
Meshkova, V., 2009. Sezonnoe razvitie khvoegryzuschykh nasekomykh [Seasonal development of foliage browsing insects]. Planeta-Print, Kharkov (in Russian).
Monastyrskij, A., Gorbatovskij, V., 1991. Massovoe razvedenie nasekomykh dlya biologicheskoi zaschity rastenji [Mass breeding of insects for biological protection of the plants]. Agropromizdat, Moscow (in Russian).
Morales-Ramos, J.A., Rojas, M.G., Shapiro-Ilan, D.I. (eds.), 2013. Mass production of beneficial organisms: Invertebrates and entomopathogen. Academic Press.
Ostapenko, L., Zlotin, A., 2000. Novyi sposob otbora, vysokozhyznesposobnykh gusenitz tutovogo shelkopryada po reaktzii khemotaksisa [A new method of selection high viability silkworm caterpillars by chemotaxis reaction]. The Kharkov Entomological Society Gazette 8(1), 73–75 (in Russian).
Petrova, N., Zlotin, O., Zaliznyak, N., 1999. Wplyw kilkosti kokoniw-glukhariw u sim’yakh na biologichni pokaznyki dochirniogo pokolinnya [The impact of the number of cocoon-grouse in families on biological indicators of daugher’s generation]. Showkiwnitztwo 22, 96–98 (in Ukrainian).
Polivtsev, O.F., Gulij, V.V., 1986. Biophysicheskie metody kontrolya kachestva nasekomykh pri ikh massovom razvedenii na IRS [Biophysical methods for quality control of insects in their mass rearing on IRS]. First All-Union Conference of Mass Rearing of Insects. Moscow State University, Moscow, pp. 17–18 (in Russian).
Raubenheimer, D., Rothman, J.M., 2013. Nutritional ecology of entomophagy in humans and other primates. Annu. Rev. Entomol. 58, 141–160.
Reshtnyak, D.Y., 2015. Morfologicheskaya izmenchivost' gemotsitov Harpalus rufipes (Coleoptera, Carabidae) pri pitanii kormami rastitel'nogo i zhivotnogo proiskhozhdeniya [Morphological variability of Harpalus rufipes (Coleoptera, Carabidae) hemocytes with regard to food of vegetable and animal origin]. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University 5(1), 133–144 (in Russian).
Safonova, T., 2002. Wdoskonalennya metodiw otzinky khybrydiw showkowychnogo showkopryada w umowakh Ukrainy [Improving the methods of evaluation of the silkworm hybrids in Ukraine]. Kharkiw (in Ukrainian).
Saltykova, E., Bienkowskaya, G., Nikolenko, A., 2007. Vnutrividovye razlichiya v mekhanizmakh formirivaniya zaschytnykh protzessov u medonosnoj pchely Apis melifera [Intraspecific differences in the mechanisms of formation of protective processes of the honeybee Apis melifera]. Journal of Evolutionary Biochemistry and Physiology 43(2), 162–167 (in Russian).
Samokhvalova, G., 1980. Vliyanie faktorov vneshnei sredy na proyavlenie nasledstvennykh osobennostei organizmov, ikh adaptatzyi i effect selektzii [Influence of environmental factors on the expression of hereditary characteristics of organisms, their adaptation and selection effects]. Uspekhi Sovremennoi Biologii 90(3), 447–461 (in Russian).
Smith, R.H., von Borstel, R.C., 1972. Genetic control of insect population. Science 178, 1164–1174.
Svyrydchenko, A.O., Brygadyrenko, V.V., 2014. Trophic preferences of Rossiulus kessieri (Diplopoda, Julidae) for the litter of various tree species. Folia Oecol. 41(2), 202–212.
Tamarina, N., 1987. Tekhnicheskaya entomologiya – novaya otrasl’ prikladnoi entomologii [Technical entomology – a new branch of applied entomology]. Results of Science and Technology. Ser. Entomology. VINITI, Moscow (in Russian).
Vorontzova, N., 1984. Biologicheskie osobennosti zernowoi moli v laboratornykh populatziyakh [Biological features of the grain moth populations in laboratory]. In: The mass breeding of insects. Chisinau: Shtiintsa, pp. 52–55 (in Russian).
Zlotin, A., 1981. Teoreticheskoe obosnowanie massovogo razvedeniya nasekomykh [Theoretical substantiation of the mass rearing of insects]. Entomological Review 60(3), 494–510 (in Russian).
Zlotin, A., Boychuk, Y., 1997. Otbor iskhodnogo materiala dlya laboratornogo kultivirovaniya nasekomykh [Selection of the starting material for the laboratory cultivation of the insects]. Vestnik Zoologii 31(4), 89–93 (in Russian).
Zlotin, A., Boychuk, Y., Markina, T., Golovko, V., Kazmiruk, V., Young, Y., Ostapenko, L., 2000. Neparnyi showkopryad u pryrodi i laboratorii [Gypsy moth in nature and laboratory: manual]. Original, Kharkov (in Ukrainian).
Zlotin, A., Chepurnaya, N., 1994. Obschie printzipy kontrolya kachest¬wa kultur nasekomykh [General principles of quality control insect cultures]. Entomological Review 73(1), 195–199 (in Russian).
Zlotin, O., Markina, T., 2009. Prawilo zalezhnosti intensywnosti proyawu taksysiw vid zhyttezdatnosti populatzij na prykladi komakh [The rule of dependence of taxes demonstration on populations viability on example of insects]. Reports of the National Academy of Sciences of Ukraine 1, 137–139 (in Ukrainian).
Zub, O., 2004. Optymizatziya fiziologichnogo stanu kultury showko¬wychnogo showkopryadu [Optimization of the physiological state of culture silkworm]. Harmony, Kharkov (in Ukrainian).
Zub, O., Zlotin, O., Ostapenko, L., 2004. Wuwchennya zalezh-nosti mizh intensywnistyu reaktzii khemotaksysu gusenytz’ i stupenem chutlywosti imago-samtziw showkowychnogo shovkopryadu do statevogo feromonu samytz [The study the relationship between chemotaxis intensity of caterpillars and sensitivity silkworm males to females ex pheromone]. Bulletin of Kharkiv National Agrarian University 5, 24–27 (in Ukrainian).
Published
2016-03-04
Section
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