Research of the plant’s functionality state during pulsed light stimulation


  • D. V. Chernetchenko Oles Honchar Dnipropetrovsk National University
  • M. P. Motsnyj Oles Honchar Dnipropetrovsk National University
  • N. P. Botsva Oles Honchar Dnipropetrovsk National University
  • О. V. Elina Oles Honchar Dnipropetrovsk National University
Keywords: bioelectrical potentials’ recording, automated physical experiment, mathematical cell model, light-induced biopotentials

Abstract

Effective and controlled plants development in artificial conditions today represents an urgent problem. Registration of bioelectrical potentials of the plants is still the best way of evaluation and diagnostics of the physiological functionality state of the biosystem. Very important role is played by an opportunity of continuous and automated control of parameters of vital activity support. This work employs hardware and software system for automated recording of bioelectrical potentials on the basis of USB-device with subsequent processing of signals on PC. We proposed the universal pattern of bioelectrical potentials’ recording which allowed to detect the response of the biological object to photo-stimulation. It can be used for the deeper understanding and investigation of biological mechanisms of electrical potentials’ generation in cells. During the experiments, we change two main parameters: the wavelength and intensity of light. System for bioelectrical potentials’ recording consists of hardware and software parts. Integrated system of recording and biometrical processing was used for analysis of the leaves’ electrical responses to photo stimuli. The dynamics of these potentials was studied, with the quantitative analysis of intensity of light level, where we obtained the minimum and maximum of sensitivity to the light intensity. As a result of modeling, we determined that electrical response of cells to photo-stimulation was based on selective conductivity of cell membrane for Н+ ions. Therefore, we showed the biophysical relation of plant light-induced potentials to the intracellular biophysical mechanisms

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Published
2014-10-11
Section
Articles

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