Models of intracellular mechanisms of plant bioelectrical potentials caused by combined stimulation
AbstractThis paper deals with bioelectrical potentials of the plants recorded during different types of stimuli and combined stimulus as well. All registrations were observed on the leaves of the corn. We used different stimuli, such as cold, heat, photo- and electrical stimulation, and certain combination of this stimuli. Hardware and software system for automated recording of bioelectrical potentials has been successfully used in this work. We proposed the universal pattern of bioelectrical potentials’ recording which allowed to detect the response of the biological object to different stimuli and various combinations of these stimuli. This pattern can be used for the deeper understanding of biological mechanisms of electrical potentials’ generation in cells and discovering of processes of accommodation of whole organisms to these stimuli. Integrated system of recording and biometrical processing was used for analysis of corn leaves electrical responses to the thermal stimuli. The dynamics of these potentials was studied, with the quantitative analysis of the potential level stabilization.We calculated the ratio of amplitude of response potentials to the first response amplitude. Mathematical models of the plant cell were used for studying of intracellular mechanisms of biopotentials gereration. As a result of modeling, we revealed that electrical response of the cells was based on selectiveconductivity of cell membrane for Н+ and Ca2+ ions. Therefore, we showed the biophysical relation of plant potentials to underlying intracellular biophysical mechanisms during thermal and combined stimulation.
Davies, E., 1987. Action potentials as multifunctional signals in plants – a unifying hypothesis to explain apparently disparate wound responses. Plant Cell Environ. 10, 623–631. >> doi.:10.1111/j.1365-3040.1987.tb01844.x
Davies, E., 2004. New functions for electrical signals in plants. New Phytol. 161, 607–610. >> doi.:10.1111/j.1469-8137.2003.01018.x
Eccles, J., 1966. Fiziologiia sinapsov [Physiology of synapses]. Mir, Moscow (in Russian).
Friesen, W.O., Friesen, J.A., 1994. NeuroDynamix: Computer models for neurophysiology. Oxford University Press, New York.
Goldman, D.E., 1943. Potential, impedance, and rectification in membranes. J. Gen. Physiol. 27(1), 37–60. >> doi.:10.1085/jgp.27.1.37
Grams, T.E.E., Lautner, S., Felle, H.H., Matyssek, R., Fromm, J., 2009, Heat-induced electrical signals affect cytoplasmic and apoplastic pH as well as photosynthesis during propagation through the maize leaf. Plant Cell Environ. 32(4), 319–326. >> doi.:10.1111/j.1365-3040.2008.01922.x
Heldt, H.W., 1997. Plant biochemistry & molecular biology. Oxford University Press, Oxford.
Hille, B., 2001. Ionic channels of excitable membranes. Sinauer Associates, Sunderland.
Hodgkin, A.L., Huxley, A.F., 1952. A quantitative description of membrane current and its application to conduction and excitation in nerve. J. Physiol. 117, 500–544.
Hoppensteadt, F.C., 1986. An introduction to the mathematics of neurons. Cambridge University Press, Cambridge.
Huguenard, J., McCormick, D.A., 1994. Electrophysiology of the neuron: An interactive neuron. Oxford University Press, Oxford.
Koch, C., Segev, I., 1989. Methods in neuronal modeling: From synapses to networks. Bradford Book, The MIT Press, Cambridge.
Lysikov, V.N., 2001. Studying some features of maize genetics and developmental biology using electrophysiological techniques. Action potentials in maize sieve tubes change phloem translocation. J. Exp. Bot. 45(4), 463–469.
Motsnyj, M.P., Elina, E.V., Vlasova, S.V., 2004. Issledovanie reakcii rasteniy, vizvannoi ritmicheskoy stimulyaciei [The study plants responses induced by repetitive stimulation]. Nauka ta osvita, Odessa 55, 37–38 (in Russian).
Muyskens, M.A., Glass, S.V., Wietsma, T.W., Gray, T.M., Mark, A., 2007. Data acquisition in the chemistry laboratory using LabVIEW software. J. Chem. Educ. 73(12), 1112–1114.
Ogren, P.J., Jones, T.P., Paul, J., 2006. Laboratory interfacing using the LabVIEW software package. J. Chem. Educ. 73(12), 1115–1116.
Rinzel, J., Ermentrout, G.B., 1989. Analysis of neural excitability and oscillations. The MIT Press, Cambridge.
Roblin, G., 1985. Analysis of the variation potential induced by wounding in plants. Plant Cell Physiol. 26, 255–261.
Rosljakova, T.V., Molchan, O.V., Vasekina, A.V., Lazareva, E.M., Sokolik, A.I., Jurin, V.M., de Bur, A.H., Babakov, A.V., 2011. Soleustojchivost’ jachmenja: Vzaimosvjaz’ jekspressii izoform vakuoljarnogo Na+/H+-antiportera s nakopleniem 22Na+ [Salt tolerance of barley: The relationship isoform expression vacuolar Na+/H+-antiporter with accumulation of 22Na+]. Fiziologija Rastenij 58(1), 28–39 (in Russian).
Stankovic, B., Witters, D.L., Zawadzki, T., Davies, E., 1998. Action potentials and variation potentials in sunﬂower: An analysis of their relationships and distinguishing characteristics. Physiol. Plantarum 103, 51–58. >> doi.:10.1034/j.1399-3054.1998.1030107.x
Thiel, G., Homann, U., Plieth, C., 1997. Ion channel activity during the action potential in Chara: A new insight with new techniques. J. Exp. Bot. 48, 609–622. >> doi.:10.1093/jxb/48.Special_Issue.609
Zhu, J.K., 2002. Salt and drought stress signal transduction in plants. Rev. Plant. Physiol. Plant. Mol. Biol., 53, 247–273. >> doi.:10.1023/A:1013383120392
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