Artificial fertilization in corn and prospects of its use


  • T. M. Satarova Agricultural Steppe Zone Institute of the National Academy of Agrarian Sciences of Ukraine

Abstract

Summarizing article is devoted to the results and perspectives of artificial fertilization in vitro in maize. The methods of purification of male and female gametes, their fusion, production of endosperm and zygotes, which are able to produce the embryo, are described. The possibilities of the combination of the transgenic technique and the technique of the artificial fertilization are estimated.

References

1. Cass D. Structure and properties of sperm cells isolated from the pollen of Zea mays / D. Cass, G. Fabi // Can. J. Bot. – 1988. – Vol. 66. – P. 819–825.
2. Chabond A. Generative cells and male gametes: isolation, physiology and biochemistry / A. Chabond, R. Perez // Int. Rev. Cytol. – 1992. – Vol. 140. – P. 205–232.
3. Control of early seed development / A. Chaudhury, A. Koltunow, T. Payne et al. // Ann. Rev. Cell. Dev. Biol. – 2001. – Vol. 17. – P. 677–699.
4. Differential gene expression between cross-fertilized and self-fertilized kernels during the early stages of seed development in maize / F. Meng, Z. Ni, L. Wu, Q. Sun // Plant Science (Oxford). – 2005. – Vol. 168. – P. 23–28.
5. Endosperm development after fusion of isolated, single maize sperm and central cells in vitro / E. Kranz, P. von Wiegen, H. Quader, H. Lörz // Plant Cell. – 1998. – Vol. 10. – P. 511–542.
6. Fertilization in maize indeterminate gametophyte 1 mutant / F. Guo, B. Q. Huang, Y. Han, S. Y. Zee // Protoplasma. – 2004. – Vol. 223. – P. 111–120.
7. Identification of genes that are up- or down-regulated in the apical or basal cell of maize two-celled embryos and monitoring their expression during zygote development by a cell manipulation and PCR-based approach / T. Okamoto, S. Scholten, H. Loerz, E. Kranz // Plant and Cell Physiology. – 2005. – Vol. 46. – P. 332–338.
8. Identification of major proteins in maize egg cells / T. Okamoto, K. Higuchi, T. Shinkawa et al. // Plant and Cell Physiology. – 2004. – Vol. 45. – P. 1406–1412.
9. In vitro pollination and fertilization in maize (Zea mays L.): technical procedures and prospects for the dissection of the double fertilization process / J. Faure, C. Digonnet, H. Mol et al. // Plant Sci. – 1994. – Vol. 104. – P. 1–10.
10. Isolated maize zygotes mimic in vivo embryonic development and express microinjected genes when cultured in vitro / N. Leduc, E. Matthys-Rochon, M. Rougier et al. // Dev. Biol. – 1996. – Vol. 177. – P. 190–203.
11. Isolation and microinjection of active sperm nuclei into egg cells and central cells of isolated embryo sacs / R. Matthys-Rochon, P. Mol, P. Heizmann, C. Dumas // Zygote. – 1994. – Vol. 2. – P. 29–35.
12. Isolation and viability of sperm cells from corn (Zea mays) and rape (Brassica oleracea) pollen grains / P. Roeckel, I. Dupuis, S. Detchepare et al. // Plant sperm cells as tools for biotechno logy. – Wageningen: Pudoc, 1988. – P. 105–110.
13. Karyogamy after electrofusion of single egg and sperm cell protoplasts from maize: cytological evidence and time course / J. Faure, H. Mogensen, C. Dumas et al. // Plant Cell. – 1993. – Vol. 5. – P. 747–755.
14. Kranz E. In vitro fertilization of single isolated gametes of maize mediated by electrofusion / E. Kranz, J. Bautor, H. Lörz // Sex. Plant Reprod. – 1991. – Vol. 4. – P. 12–16.
15. Kranz E. In vitro fertilization with isolated, single gametes results in zygotic embryogenesis and fertile maize plants / E. Kranz, J. Bautor, H. Lörz // Plant Cell. – 1993. – Vol. 5. – P. 739–746.
16. Kranz E. Early cytological events after induction of cell division in egg cells and zygote development following in vitro fertilization with angiosperm gametes / E. Kranz, P. von Wiegen, H. Lörz // Plant J. – 1995. – Vol. 9. – P. 9–23.
17. Lin X. D. Changes and localization of binding protein (BiP) during maize embryo development / X. D. Lin, J. R. Fu, S. Z. Huang // Journal of Tropical and Subtropical Botany. – 2004. – Vol. 12. – P. 159–162.
18. Matthys-Rochon E. Characterization of Zea mays embryo sac using fluorescent probes and microinjection of Lucifer yellow into the female cells / E. Matthys-Rochon, C. Digonnet, C. Dumas // Biotechnology applications of microinjection, microscopic imaging and fluorescence. – NY.: Plenum Press, 1992. – P. 53–60.
19. Mol R. Embryogenesis and plant regeneration from maize zygotes by in vitro culture of fertilized embryo sacs / R. Mol, R. E. Matthys-Rochon, C. Dumas // Plant Cell Rep. – 1995. – Vol. 14. – P. 743–747.
20. Procedure to isolate viable sperm cells from corn (Zea mays L.) pollen grains / I. Dupuis, I. Roeckel, E. Matthys-Rochon, C. Dumas // Plant Physiol. – 1987. – Vol. 85. – P. 876–878.
21. Qiu Y. L. In vitro fertilization as a technique platform used in the research of sexual reproduction of angiosperms / Y. L. Qiu, M. Z. Zheng, H. Q. Tian // Journal of Tropical and Subtropical Botany. – 2003. – Vol. 11. – P. 290–296.
22. Raghavan V. One hundred years of zygotic embryo culture investigations // In Vitro Cellular and Developmental Biology Plant. – 2003. – Vol. 39. – P. 437–442.
23. Regeneration of fertile barley plants from mechanically isolated protoplasts of the fertilized egg cell / P. Holm, S. Knudsen, P. Mouritzen et al. // Plant Cell. – 1994. – Vol. 6. – P. 531–543.
24. Roeckel P. Survival at 200C and cryopreservation of isolated sperm cells from Zea mays pollen grains / P. Roeckel, C. Dumas // Sex. Plant Reprod. – 1993. – Vol. 6. – P. 212–216.
25. Saze H. Maintenance of CpG methylation is essential for epigenetic inheritance during plant gametogenesis / H. Saze, O. M. Scheid, J. Paszkowski // Nature Genetics. – 2003. – Vol. 34. – P. 65–69.
26. Scholten S. In vitro fertilization and expression of transgene in gametes and zygotes / S. Scholten, E. Kranz // Sex. Plant Reprod. – 2001. – Vol. 14. – P. 35–40.
27. Ultrastructural characterization and three dimensional reconstruction of isolated maize (Zea mays L.) egg cell protoplasts / J. Faure, H. Mogensen, E. Kranz et al. // Protoplasma. – 1992. – Vol. 171. – P. 97–103.
28. Wagner V. Morphometric analysis of isolated Zea mays sperms / V. Wagner, C. Dumas, H. Mogensen // J. Cell Sci. – 1989. – Vol. 93. – P. 179–184.
29. Zhang G. RNA and protein synthesis in sperm cells isolated from Zea mays L. pollen / G. Zhang, D. Gifford, D. Cass // Sex. Plant Reprod. – 1993. – Vol. 6. – P. 239–243.
Published
2006-02-21
Section
Articles