Alimentary triggers of hormone dependent breast cancers

  • T. Y. Lykholat Oles Honchar Dnipropetrovsk National University
  • О. А. Lykholat Academy of Customs Service of Ukraine
  • V. S. Nedzvetsky Oles Honchar Dnipropetrovsk National University
  • V. V. Laktionov Central Hospital Munisipal Enterprise
Keywords: exogenous estrogens, lipid peroxidation, antioxidant protection system


Breast cancer (BC) consistently holds the leading positions in the structure of morbidity and mortality of the female population. Food containing veterinary hormones is extremely dangerous to human health: estrogens are female sex hormones. Excessive level of estrogen in the body gives rise to diseases of varying severity: in women (especially of older age) it may cause breast cancer. The paper investigates the processes of lipid peroxidation and the status of antioxidant protection system in rats of different ages exposed to exogenous estrogens. The purpose of the work is to study lipid peroxidation and antioxidative protection status in rats of different ages exposed to exogenous estrogens for determining the trigger mechanisms for tumor development. Experiments were conducted on female Wistar rats exposed to exogenous estrogen for 45 days. At the beginning of the experiment, age of experimental animals was 3 months in pubertal period and 6 months as mature ones. The control groups consisted of intact animals of appropriate age. To simulate the influence of exogenous estrogen, rats’ food was treated with the Sinestron drug at the rate of 2 mg per kg. The research materials were serum and liver of rats. Objects of the research were indicators of lipid peroxidation activity (content of TBA-active products) and antioxidant protection system (reduced glutathione (RG) level), glutathione transferase (GT), glutathione reductase (GR), glutathione peroxidase (GP), superoxide dismutase (SOD) activity, and total antioxidative activity (AOA). Data obtained was treated with standard methods of estimation of variation series. Various degrees of peroxidation intensification depending on the age and organs were determined. Maximum excess of control indexes in the serum was observed and it indicated synthetic estrogen effect of on all major body systems. In prepubertal period females’ liver the reaction of prooxidant system and tension in the antioxidant protection system exceeded the strength of response in the body of mature animals. The organ discreteness of changes in the activity of antioxidant protection enzymes depending on the age of the animals was recorded. Given the involvement of glutathione system in the deactivation of estrogens through their conjugation in reactions catalyzed by glutathione transferase, decreased enzyme activity may lead to accumulation of highly active intermediate metabolites with subsequent damage to intracellular structures. Later on, these phenomena may become a trigger of reduction of the compensatory mechanisms’ potential, which together with the genotoxic effect of exogenous estrogens is an important pathogenetic element in carcinogenesis: they initiate the development of proliferative processes and occurrence of cancer conditions, in particular, hormone dependent breast tumors, in future. 


Alyaev, Y.G., Bezrukov, E.A., Tsestiperov, P.A., 2006. Molekulyarnaya patologia raka pretstatel’noy gelezy: Diagnostitcheskaya i prognostitcheskaya znatchimos’t osnovnych markerov [Molecular pathology of gland cancer: Diagnostic and prognostic significance of major markers]. Oncourology 2, 45–50.
Baena Ruiz, R., Salinas Hernández, P., 2013. Diet and cancer: Risk factors and epidemiological evidence. Maturitas 13, 360–365. >> dx.doi:10.1016/j.maturitas.2013.11.010
Boucher, B.A., Cotterchio, M., Curca, I.A., Kreiger, N., Harris, S.A., Kirsh, V.A., Goodwin, P.J., 2012. Intake of phytoestrogen foods and supplements among women recently diagnosed with breast cancer in Ontario, Canada. Cancer. Prev. Control 4(3), 95–107. >> doi:10.1080/01635581.2012.687426
Bouwens, C.S., van Rensburg, S.J., de Kock, L., Apffelstaedt, L.P., Kotze, M.J., 2011. Influence of genetic factors on the development of breast cancer in the older woman. Reprod. Toxicol. 3, 302–311. >> DOI: 10.2174/1874609811205020140
Brinkman, M.T., Baglietto, L., Krishnan, K., English, D.R, Severi, G., Morris, H.A., Hopper, J.L, Giles, G.G., 2010. Consumption of animal products, their nutrient components and postmenopausal circulating steroid hormone concentrations. Eur. J. Clin. Nutr. 64, 176–183.
Brynes, S.D., 2005. Demystifying 21 CFR Part 556 tolerances for residues of new animal drugs in food. Regul. Toxicol. Pharmacol. 42(3), 324–327.
Chun, J., Pocock, B., Joseph, K.A., Tamer, E.M., Klein, L., Schnabel, F., 2012. Breast cancer risk factors in younger and older women. Curr. Aging. Sci. 5(2), 140–147. >> doi:10.1245/s10434-008-0176-8
Fedorenko, Z.P., Myhaylovich, Y.Y., Gulak, L.O., 2013. Rak v Ukrajini [Cancer in Ukraine] Bulletin of National Cancer-Register of Ukraine 2011–2012 (in Ukrainian).
Fung, T.T., Hu, F.B., McCullough, M.L., Newby, P.K., Willett, W.C., Holmes, M.D., 2006. Diet quality is associated with the risk of estrogen receptor-negative breast cancer in postmenopausal women. J. Nutr. 136, 466–472.
Gavrilov, V.B., Gavrilova, A.P., Mazhul’, L.M., 1987. Analiz metodov opredeleniya produktov perekisnogo okisleniya lipidov v cyvorotke krovi po testy s tiobarbiturovoy kislotoy [Analysis of methods for determining lipid peroxidation products in the blood serum on test with thiobarbituric acid]. Vopr. Med. Chim. 1, 118–122.
Klebanov, G.I., 1988. Otsenka antiokislitel’noy aktivnosti plazmy krovi s primeneniem geltochnyuch lipoproteidov [Evaluation of antioxidative activity of blood plasma using yolk lipoproteins]. Lab. Delo 5, 59–62 (in Russian).
Larrea, F., Chirinos, M., 2007. Impact on human health of hormonal additives used in animal production. Rev. Invest. Clin. 59(3), 206–211.
Lykholat, T.Y., 2012. Zalezghnist’ protsesiv metastazuvannya vid retseptornogo profilyu ta viku chvorych n arak molotchnoy galozy [The dependence of the process of metastasis from receptor profile and age of patients with breast cancer]. Vіsn. Lviv. Univ. Bіol. 56, 242–247 (in Ukrainian).
Lykholat, T.Y., Antonyuk, S,V., Probatchay, V.I., 2013. Osoblyvosti receptornoi' vidpovidi chynnykiv zrostannja puhlyny u hvoryh na rak molochnoi' zalozy [Features of receptor response of factors of tumor growth in patients with breast cancer]. Vìsn. Dnìpropetr. Unìv. Ser. Bìol. Med. 3(1), 38–43 (in Ukrainian).
Mariani, A., Jeandel, C., Paris, F., Ecochard, R., 2011. Puberty and pubertal growth dynamics in children with idiopathic short stature. J. Pediatr. Endocrinol. Metab. 24(6), 319–325.
Owens, C.W.I., Belcher, R.V., 1965. A colorimetric micromethod for determination of glutathione. Biochem. J. 94(3), 705–711.
Pereslegina, I.A. 1989. Aktivnost’ antioxidantnyuch fermentov clunyu zdorovych detey [The activity of antioxidant enzymes saliva of healthy children]. Lab. delo 11, 20–23 (in Russian).
Smolanka, I.I., Sklyar, S.Y., Golovko, T.S., Ganuch, O.V., 2013. Ckryning, profilaktica ta rannya diagnostika raku grudnoy zhelezy [Screening, prevention and early detection of breast cancer]. Clin. Oncol. 8(1), 12–25 (in Ukrainian).
Stephany, R.W., 2001. Hormones in meat: Different approaches in the EU and in the USA. Handb. Exp. Pharmacol. 103, 357–363. >> doi:10.1111/j.1600-0463.2001.tb05787.x
Stephany, R.W., 2010. Hormonal growth promoting agents in food producing animals. Handb. Exp. Pharmacol. 195, 355–367. >> doi:10.1007/978-3-540-79088-4_16
Wang, S., Wang, X.H., 2007. Analytical methods for the determination of zeranol residues in animal products: A review. Food Addit. Contam. 24(6), 573–582.

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