Biomarkers of metabolism disturbance in bivalve molluscs induced by environmental pollution with processed by-products of oil


  • E. V. Sukharenko Kerch State Marine Technology Iniversity Bingol University
  • V. S. Nedzvetsky Bingol UniversityOles Honchar Dnipro National University http://orcid.org/0000-0003-1969-1182
  • S. V. Kyrychenko Oles Honchar Dnipro National University
Keywords: bivalve mollusks, oil products, oxidative stress, antioxidant enzime activity

Abstract

Processed by-products of oil are the most common pollutants in all river and sea water. The increase in oxidative stress in bivalve molluscs was studied in both tissues of the hepatopancreas and the gill. The model for artificial treatment with processed by-products of oil was performed in a laboratory experiment with the river mollusc Dreissena polymorpha Pallas, 1771. The exposure of the molluscs over 28 days to mazut 50 mg/l induced significant increase of both final product of lipid peroxidation (LPO) and antioxidant enzime activity. A significant increase in LPO was observed in the hepatopancreas and gill of D. polymorpha treated with mazut compared to the control group. Antioxidant enzyme activity of cartalase, supeoxide dismutase, glutathione reductase and glutathione-S-transferase showed a greater increase (by almost 1.5 times) in the hepatopancreas than in the gill of D. polymorpha. A similar LPO growth and modulation of antioxidant enzyme activity were determined in the hepatopancreas and gill of the mussel Mytilus galloprovincialis Lamarck, 1879 collected in an area polluted with resins, hydrocarbons and asphaltenes, Donuzlav lake in the Kerch gulf. Varied cellular reactivation of the antioxidant enzyme system in the hepatopancreas rather than the gill was observed in both kinds of mollusc Dreissena and Mytilus. The obtained results are evidence of the higher sensitivity of the hepatopancreas cells of bivalve molluscs to organic pollutants compared to the gill cells.

References

Abderrahim, M., Arribas, S. M., & Condezo-Hoyos, L. (2017). A novel pyrogallol red-based assay to assess catalase activity: Optimization by response surface methodology. Talanta, 166(1), 349–356.
Beyer, W. F., Jr, & Fridovich, I. (1987). Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Analytical Biochemistry, 161, 559–566.
Bocchetti, R., Fattorini, D., Pisanelli, B., Macchia, S., Oliviero, L., Pilato, F., Pellegrini, D., & Regoli, F. (2008). Contaminant accumulation and biomarker responses in caged mussels, Mytilus galloprovincialis, to evaluate bioavailability and toxicological effects of remobilized chemicals during dredging and disposal operations in harbour areas. Aquatic Toxicology, 89(4), 257–266.
Cappello, T., Maisano, M., D'Agata, A., Natalotto, A., Mauceri, A., Fasulo, S. (2013). Effects of environmental pollution in caged mussels (Mytilus galloprovincialis). Marine Environmental Researchs, 91, 52–60.
Chatel, A., Hamer, B., Talarmin, H., Dorange, G., Schroder, H. C., Muller, W. E. (2010). Activation of MAP kinase signaling pathway in the mussel Mytilus galloprovincialis as biomarker of environmental pollution. Aquatic Toxicology, 96(4), 247–255.
Damiens, G., Gnassia-Barelli, M., Loques, F., Romeo, M., & Salbert, V. (2007). Integrated biomarker response index as a useful tool for environmental assessment evaluated using transplanted mussels. Chemosphere, 66(3), 574–583.
Fernandez, B., Campillo, J. A., Martinez-Gomez, C., & Benedicto, J. (2010). Antioxidant responses in gills of mussel (Mytilus galloprovincialis) as biomarkers of environmental stress along the Spanish Mediterranean coast. Aquatic Toxicology, 99(2), 186–197.
Fiskum, G., Danilov, C. A., Mehrabian, Z., Bambrick, L. L., Kristian, T., McKenna, M. C., Hopkins, I., Richards, E. M., & Rosenthal, R. E. (2008). Postischemic oxidative stress promotes mitochondrial metabolic failure in neurons and astrocytes. Annals of the New York Academy of Sciences, 1147, 129–138.
Franco, L., Romero, D., Garcia-Navarro, J. A., Teles, M., & Tvarijonaviciute, A. (2016). Esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) in gills of Mytilus galloprovincialis exposed to pollutants: Analytical validation and effects evaluation by single and mixed heavy metal exposure. Marine Pollution Bulletin, 102(1), 30–35.
Gomiero, A., Da Ros, L., Nasci, C., Meneghetti, F., Spagnolo, A., & Fabi, G. (2011). Integrated use of biomarkers in the mussel Mytilus galloprovincialis for assessing off-shore gas platforms in the Adriatic Sea: Results of a two-year biomonitoring program. Marine Pollution Bulletin, 62(11), 2483–2495.
Gorbi, S., Pellegrini, D., Tedesco, S., & Regoli, F. (2004). Antioxidant efficiency and detoxification enzymes in spotted dogfish Scyliorhinus canicula. Marine Environmental Research, 58(2–5), 293–297.
Guidi, P., Frenzilli, G., Benedetti, M., Bernardeschi, M., Falleni, A., Fattorini, D., Regoli, F., Scarcelli, V., & Nigro, M. (2010). Antioxidant, genotoxic and lysosomal biomarkers in the freshwater bivalve (Unio pictorum) transplanted in a metal polluted river basin. Aquatic Toxicology, 100(1), 75–83.
Gunzler, W. A., Flohe, L., & Greenwald, E. R. (1985). Glutathione Peroxidase. In: CRC handbook of methods for oxygen radical research. CRC Press, Boston.
Jarque, S., Prats, E., Olivares, A., Casado, M., Ramon, M., & Pina, B. (2014). Seasonal variations of gene expression biomarkers in Mytilus galloprovincialis cultured populations: Temperature, oxidative stress and reproductive cycle as major modulators. Science of the Total Environment, 499, 363–372.
Lacroix, C., Richard, G., Seguineau, C., Guyomarch, J., Moraga, D., & Auffret, M. (2015). Active and passive biomonitoring suggest metabolic adaptation in blue mussels (Mytilus spp.) chronically exposed to a moderate contamination in Brest harbor (France). Aquatic Toxicology, 162, 37–126.
Lopes, B., Ferreira, A. M., & Bebianno, M. J. (2012). Responses of CYP450 de-pendent system to aliphatic and aromatic hydrocarbons body burden in tran-splanted mussels from South coast of Portugal. Ecotoxicology, 21, 730–749.
Martins, M., Costa, P. M., Raimundo, J., Vale, C., Ferreira, A. M., & Costa, M. H. (2012). Impact of remobilized contaminants in Mytilus edulis during dredging operations in a harbour area: Bioaccumulation and biomarker responses. Ecotoxicology and Environmental Safety, 85, 96–103.
Novitsky, R. A., Sukharenko, E. V., Nedzvetsky, V. S. (2013). Molecular biomar-kers of Al3+ ions effect on oxidative stress generation and cell reactivity in fish Lepomis gibbosus (Pisces: Centrarchidae). Hydrobiological Journal, 49(6), 65–75.
Ohkawa, H., Ohishi, N., & Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95(2), 351–358.
Orbea, A., Ortiz-Zarragoitia, M., Sole, M., Porte, C., & Cajaraville, M. P. (2002). Antioxidant enzymes and peroxisome proliferation in relation to contaminant body burdens of PAHs and PCBs in bivalve molluscs, crabs and fish from the Urdaibai and Plentzia estuaries (Bay of Biscay). Aquat Toxicology, 58, 75–98.
Ozkan, D., Dagdeviren, M., Katalay, S., Guner, A., & Yavaşoglu, N. U. (2017). Multi-biomarker responses after exposure to pollution in the mediterranean mussels (Mytilus galloprovincialis L.) in the Aegean Coast of Turkey. Bulletin of Environmental Contamination and Toxicology, 98(1), 46–52.
Regoli, F., Nigro, M., Chierici, E., Cerrano, C., Schiapparelli, S., Totti, C., & Bavestrello, G. (2004). Variations of antioxidant efficiency and presence of endosymbiotic diatoms in the Antarctic porifera Haliclona dancoi. Marine Environmental Research, 58(2–5), 637–640.
Ruczynska, W. M., Szlinder-Richert, J., Malesa-Ciecwierz, M., & Warzocha, J. (2011). Assessment of PAH pollution in the southern Baltic Sea through the analysis of sediment, mussels and fish bile. Journal of Environmental Monitoring, 13(9), 2535–2542.
Sukharenko, E. V., Nedzvetsky, V. S., & Maximov, V. I. (2015) The characteristic of molecular processes of a biotrasformation in a gepatopankreas and gills of adults the polovozrelykh of individuals of a river dreyssena (Dreyssena polymorpha) in the conditions of pollution of the habitat. Veterinarian, 6, 27–32.
Turja, R., Hoher, N., Snoeijs, P., Barsienė, J., Butrimavicienė, L., Kuznetsova, T., Kholodkevich, S. V., Devier, M. H., Budzinski, H., & Lehtonen, K. K. (2014). A multibiomarker approach to the assessment of pollution impacts in two Baltic Sea coastal areas in Sweden using caged mussels (Mytilus trossulus). Science of The Total Environment, 473–474, 398–409.
Turja, R., Soirinsuo, A., Budzinski, H., Devier, M. H., & Lehtonen, K. K. (2013). Biomarker responses and accumulation of hazardous substances in mussels (Mytilus trossulus) transplanted along a pollution gradient close to an oil terminal in the Gulf of Finland (Baltic Sea). Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 157(1), 80–92.
Vidal-Linan, L., Bellas, J., Soriano, J. A., Concha-Grana, E., Muniategui, S., & Beiras, R. (2016). Bioaccumulation of PCB-153 and effects on molecular biomarkers acetylcholinesterase, glutathione-S-transferase and glutathione peroxidase in Mytilus galloprovincialis mussels. Environmental Pollution, 214, 885–891.
Published
2017-05-17
Section
Articles