Automated experiment for registration of bioelectrical potentials
AbstractA hardware-software complex automated system of recording bioelectrical potentials, which is based on a USB-device with subsequent processing of signals with PC was developed in this work. We proposed a universal scheme of registration of bioelectrical potentials, which allows one to detect the reaction of biological objects to different stimuli, such as cold, heat, photo- and electrical stimulation, and to different combinations of these stimuli (Motsnyj et al., 2004). They could be applied for deeper understanding of the biological mechanisms of generation of electrical potentials in cells and discovering the accommodation processes of organisms as a whole to these stimuli. The system for registration of bioelectrical potentials consists of hardware and software parts. The software part consists of the client and server sides, which transmit experimental data to the network. The client-side software renders a quantitative analysis and stores the results in a database. An integrated system of registration and biometrical processing was applied for analysis of the electrical responses of maize leaves to heat stimuli. The dynamics of these potentials were studied and a quantitative analysis of the potential level stabilization was made. We found that amplitude relation of responses to the initial response increased and stabilized at the level of 130%. Mathematical models of the plant cell for discovering intracellular mechanisms of biopotentials registration were developed. As a result of modeling, we found that the electrical response of the cells is based on selective conductance of the cell membrane for Н+ and K+ ions. By this way, we show the biophysical relation of plant potentials to intracellular biophysical mechanisms.
Davies, E., 1987. Action potentials as multifunctional signals in plants – a unifying hypothesis to explain apparently disparate wound responses. Plant Cell Environ. 10, 623–631. >> doi:10.1111/j.1365-3040.1987.tb01844.x
Eccles, J., 1966. Physiology of synapses [Fiziologiia sinapsov]. Mir, Moscow (in Russian).
Friesen, W.O., Friesen, J. A., 1994. NeuroDynamix: Computer Models for Neurophysiology. Oxford University Press, NewYork.
Goldman, D.E., 1943. Potential, impedance, and rectification in membranes. J. Gen. Physiol. 27, 37–60. >> doi:10.1085/jgp.27.1.37
Hodgkin, A.L., Huxley, A.F., 1952. A quantitative description of membrane current and its application to conduction and excitation in nerve. J. Physiol. 117, 500–544.
Hoppensteadt, F.C., 1986. An introduction to the mathematics of neurons. Cambridge University Press, Cambridge.
Hille, B., 2001. Ionic channels of excitable membranes. Sinauer Associates, Sunderland, MA.
Huguenard, J., McCormick, D.A., 1994. Electrophysiology of the neuron: An interactive neuron. Oxford University Press, Oxford, UK.
Heldt, H.W., 1997. Plant biochemistry & molecular biology. Oxford University Press, Oxford, UK.
Koch, C., Segev, I., 1989. Methods in neuronal modeling: From synapses to networks. Bradford Book, The MIT Press., Cambridge, MA.
Lysikov, V.N., 2001. Studying some features of maize genetics and developmental biology using electrophysiological techniques action potentials in maize sieve tubes change phloem translocation. J. Exp. Bot. 45(4), 463–469.
Motsnyj, M.P., Elina, E.V., Vlasova, S.V., 2004. Issledovanie reakcii rasteniy, vizvannoi ritmicheskoy stimulyaciei [The study plants responses induced by repetitive stimulation]. Nauka ta Osvita, Odessa 55, 37–38 (in Russian).
Muyskens, M.A., Glass, S.V., Wietsma, T.W., Gray, T.M., Mark, A., 2007. Data acquisition in the chemistry laboratory using LabVIEW software. J. Chem. Educ. 73(12), 1112–1114.
Ogren, P.J., Jones, T.P., Paul, J., 2006. Laboratory interfacing using the LabVIEW software package. J. Chem. Educ. 73(12), 1115–1116.
Rosljakova, T.V., Molchan, O.V., Vasekina, A.V., Lazareva, E.M., Sokolik, A.I., Jurin, V.M., de Bur, A.H., Babakov, A.V., 2011. Soleustojchivost’ jachmenja: Vzaimosvjaz’ ekspressii izoform vakuoljarnogo Na+/H+-antiportera s nakopleniem 22Na+ [Salt tolerance of barley: The relationship isoform expression vacuolar Na+/H+-antiporter with accumulation of 22Na+]. Fiziologija Rastenij 58(1), 28–39 (in Russian).
Roblin, G., 1985. Analysis of the variation potential induced by wounding in plants. Plant Cell Physiol. 26, 255–261.
Rinzel, J., Ermentrout, G.B., 1989. Analysis of neural excitability and oscillations. MIT Press, Cambridge, MA.
Stankovic, B., Witters, D.L., Zawadzki, T., Davies, E., 1998. Action potentials and variation potentials in sunﬂower: An analysis of their relationships and distinguishing characteristics. Physiol. Plantarum 103, 51–58. >> doi:0.1034/j.1399-3054.1998.1030107.x
Thiel, G., Homann, U., Plieth, C., 1997. Ion channel activity during the action potential in Chara: A new insight with new techniques. J. Exp. Bot. 48, 609–622. >>doi:10.1093/jxb/48.Special_Issue.609
Thorsten, E.E., Rainer, M., Fromm, J., 2009. Heat-induced electrical signals affect cytoplasmic and apoplastic pH as well as photosynthesis during propagation through the maize leaf. Plant Cell Environ. 32, 319–326.
Zhu, J.K., 2002. Salt and drought stress signal transduction in plants. Rev. Plant. Physiol. Plant. Mol. Biol. 53, 247–273.
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