Morphological variability of Bembidion aspericolle (Coleoptera, Carabidae) populations in conditions of anthropogenic impact

Keywords: population variability; sexual dimorphism; morphometrics; riparian beetles


Bembidion (Talanes) aspericolle (Germar, 1829) is a Western Palearctic species which lives on the shores of the Atlantic Ocean, the Mediterranean, Black and Caspian Seas and saline inland habitats from Central Europe to Central Asia. Anthropogenic impact is one of the most important environmental factors affecting the morphological variability of ground beetles. The objective of our research is assessment of the morphological variability of this species in three ecosystems differing by intensity of anthropogenic impact. 13 linear characteristics, one angular characteristic, density of pores on the prothorax and elytra, contrast of spots on the beetles’ elytra were measured, and 6 morphometric indices were calculated. The mean value of body length in females is more than in males in the studied populations. In the ecosystem with high anthropogenic pressure, female body length is shorter by 3.7% and elytra length is shorter by 6.0% than in females in the ecosystem with low anthropogenic impact. Differences between populations in the body length of males are not significant. In the ecosystem with high anthropogenic transformation, sexual dimorphism is observed only on head and prothorax width. The ratio of maximum width of elytra to maximum prothorax width decreases significantly with increasing anthropogenic load. The impact of anthropogenic factors on the ecosystem produces significant changes in elytra length and head width of B. aspericolle and in four of the six morphometric indices. It is reasonable to use these morphometric characteristics of B. aspericolle adults in bioindication. The complex of anthropogenic factors does not have a significant impact on the value of anterior and posterior angles of prothorax, density of prothorax and elytra puncturing and contrast of the light spots at the top of the elytra. The sex of the specimen influences all linear characteristics. The absence of significant differences in morphometric indices between males and females shows that the body proportions of the beetles remain unchanged and only linear dimensions vary. Research on the morphological variability of B. aspericolle is important for understanding microevolutionary processes in populations of beetles under anthropogenically induced changes in the environment.


Abdurakhmanov, G. M., Nakhibasheva, G. M., Klycheva, S. M., Magomedova, S. T., & Elderhanova, Z. M. (2010). Vidovoy sostav i geograficheskoe rasprostranenie zhuzhelits roda Bembidion Respubliki Dagestan [Species structure and geographical distribution of ground beetles of genus Bembidion of Daghestan Republic]. The South of Russia: Ecology, Development, 2, 56–61 (in Russian).
Arndt, E., & Putchkov, A. V. (1997). Phylogenetic investigation of Cicindelidae (Insecta: Coleoptera) using larval morphological characters. Zoologischer Anzeiger, 234(3–4), 231–241.
Brygadyrenko, V. V. (2016). Evaluation of ecological niches of abundant species of Poecilus and Pterostichus (Coleoptera: Carabidae) in forests of the steppe zone of Ukraine. Entomologica Fennica, 27(2), 81–100.
Brygadyrenko, V. V., & Fedorchenko, D. O. (2008). Morfologichna minlyvist’ populjacii’ Carabus hungaricus scythes (Coleoptera, Carabidae) v umovah ostrova Hortycja (Zaporiz’ka oblast’) [Morphological variability of populations Carabus hungaricus scythus (Coleoptera, Carabidae) on Кhortitsa island (Zaporizhzhya province)]. Visnyk of Dnipropetrovsk University, Biology, Ecology, 16(1), 20–27.
Brygadyrenko, V. V., & Korolev, O. V. (2015). Morphological polymorphism in an urban population of Pterostichus melanarius (Illiger, 1798) (Coleoptera, Carabidae). Graellsia, 71(1), e025.
Brygadyrenko, V. V., & Reshetniak, D. Y. (2014). Morphological variability among populations of Harpalus rufipes (Coleoptera, Carabidae): What is more important, the mean values or statistical peculiarities of distribution in the population? Folia Oecolica, 41, 109–133.
Brygadyrenko, V. V., & Reshetniak, D. Y. (2016). Morphometric variability of Clitellocephalus ophoni (Eugregarinida, Gregarinidae) in the intestines of Harpalus rufipes (Coleoptera, Carabidae). Archives of Biological Sciences, 68(3), 587–601.
Brygadyrenko, V. V., & Slynko, V. O. (2015). Morphological variability of Bembidion articulatum (Coleoptera, Carabidae) populations: Linear dimensions depend on sex, while morphological indices depend on ecosystems. International Journal of Applied Environmental Sciences, 10, 163–187.
Di Grumo, D., & Lovei, G. (2016). Body size inequality in ground beetle (Coleoptera: Carabidae) assemblages as a potential method to monitor environmental impacts of transgenic crops. Periodicum Biologorum, 118(3), 223–230.
Eremina, M. A., & Gruntenko, N. E. (2017). Neyroendokrinnaya stress-reaktsiya nasekomyih: Istoriya razvitiya kontseptsii [The neuroendocrine stress-response in insects: The history of the development of the concept]. Vavilov Journal of Genetics and Breeding, 21(7), 825–832 (in Russian).
Hanna, M. E., Bednářová, A., Rakshit, K., Chaudhuri, A., O’Donnell, J. M., & Krishnan, N. (2015). Perturbations in dopamine synthesis lead to discrete physiological effects and impact oxidative stress response in Drosophila. Journal of Insect Physiology, 73, 11–19.
Hirashima, A., Sukhanova, M. J., & Rauschenbach, I. Y. (2000). Genetic control of biogenic-amine systems in Drosophila under normal and stress conditions. Biochemical Genetics, 38(5–6), 163–176.
Hurka, K. (1996). Carabidae of the Czech and Slovak Republics. Kabourek, Zlin.
Lagisz, M. (2008). Changes in morphology of the ground beetle Pterostichus oblongopunctatus F. (Coleoptera; Carabidae) from vicinities of a zinc and lead smelter. Environmental Toxicology and Chemistry, 27(8), 1744–1747.
Makarov, K. V., & Matalin, A. V. (2009). Ground-beetle communities in the Lake Elton region, southern Russia: A case study of a local fauna (Coleoptera: Carabidae). In: Marusik, Y., & Fet, V. (Ed.). Species and communities in extreme environments. Festschrift towards the 75th anniversary and a laudatio in honour of academician Yuri Ivanovich Chernov. Pensoft Publishers & KMK Scientific Press, Sofia – Moscow. Pp. 357–384.
Matalin, A., & Makarov, M. (2011). Using demographic data to better interpret pitfall trap catches. ZooKeys, 100, 223–254.
Michailov, V. A. (2013). K faune, bioekologii i rasprostraneniyu zhestkokrylyh (Coleoptera) ostrova Dzharyilgach [On the fauna, bio-ecology and distribution of Coleoptera of Dzharylgach island]. Naukovi Zapiski Derzhavnogo Prirodoznavchogo Muzeyu, 29, 113–120 (in Russian).
Mirth, C. K., Tang, H. Y., Makohon-Moore, S. C., Salhadar, S., Gokhale, R. H., Warner, R. D., Koyama, T., Riddiford, L. M., & Shingleton, A. W. (2014). Juvenile hormone regulates body size and perturbs insulin signaling in Drosophila. Proceedings of the National Academy of Sciences, 111(19), 7018–7023.
Moskalev, A., Zhikrivetskaya, S., Krasnov, G., Shaposhnikov, M., Proshkina, E., Borisoglebsky, D., Danilov, A., Peregudova, D., Sharapova, I., Dobrovolskaya, E., Solovev, I., Zemskaya, N., Shilova, L., Snezhkina, A., & Kudryavtseva, A. (2015). A comparison of the transcriptome of Drosophila melanogaster in response to entomopathogenic fungus, ionizing, starvation and cold shock. BMC Genomics, 16(13), S8.
Müller-Motzfeld, G. (2007). Die Salz- und Küstenlaufkäfer Deutschlands – Verbreitung und Gefährdung. Angewandte Carabidologie, 8, 17–27.
Nakhibasheva, G. M., Mukhtarova, G. M., Ismailova, K. A., & Klycheva, S. M. (2011). Analiz zhiznennyih form imago zhuzhelits Tersko-Kumskoy nizmennosti Dagestana [Analyze of the living form of carabidae’s imago in Tersko-Kumsckay lowland of Dagestan]. Polythematic Online Scientific Journal of Kuban State Agrarian University, 69(5), 1–15 (in Russian).
Neri, P., Bonavita, P., Gudenzi, I., Magrini, P., & Toledano, L. (2011). Bembidiina della fauna italo-corsa: Chiavi di identificazione (Insecta Coleoptera Carabidae). Quaderno di Studi e Notizie di Storia Naturale della Romagna, 33, 1–183.
Nitzu, E. (2003). Contributions to the knowledge of the tribus Bembidiini (Coleoptera: Carabidae) from Romania. Travaux du Muséum National d’Histoire Naturelle “Grigore Antipa”, 45, 179–185.
Puchkov, O. V., & Slynko, V. O. (2011). Bembidion aspericolle (Germar, 1872). In: Paxomov, O. Y. (Ed.). Chervona knyga Dnipropetrovskoyi oblasti. Tvarynnyj svit. Novyj Druk, Dnipropetrovsk. P. 69 (in Ukrainian).
Putchkov, A. V. (2011). Ground beetles of the Ukraine (Coleoptera, Carabidae). ZooKeys, 100, 503–515.
Putchkov, A. V. (2012). Faunisticheskiy obzor karaboidnyh zhukov (Coleoptera, Caraboidea) Ukrainy [A review of the caraboids-beetles (Coleoptera, Сaraboidea) of Ukraine]. Ukrayinskiy Entomologichniy Zhurnal, 2(5), 3–44 (in Russian).
Putchkov, A. V., & Brygadyrenko, V. V. (2018). Ridkisni tverdokryli nadrodyny Caraboidea (Coleoptera, Adephaga) Dnipropetrovskoyi oblasti [Rare beetles of the Caraboidea superfamily (Coleoptera, Adephaga) in Dnipropetrovsk oblast]. Zhurfond, Dnipro.
Ratti, E. (1983). Gli elementi caratteristici della coleotterofauna dei giuncheti alofili della laguna di venezia. Società Veneziana di Scienze Naturali, 8, 37–46.
Rauschenbach, I. Y., Gruntenko, N. E., Bownes, M., Karpova, E. K., Chentsova, N. A., Sukhanova, M. Z., & Adon'eva, N. V. (2003). Ecdysteroids and juvenile hormone control the early and late stages of oogenesis, respectively, during stress in Drosophila. Doklady Biological Sciences, 389, 127–129.
Rauschenbach, I. Y., Sukhanova, M. Z., Hirashima, A., Sutsugu, E., & Kuano, E. (2000). Role of the ecdysteroid system in the regulation of Drosophila reproduction under environmental stress. Doklady Biological Sciences, 375, 641–643.
Sarup, P., Sørensen, P., & Loeschcke, V. (2014). The long-term effects of a life-prolonging heat treatment on the Drosophila melanogaster transcriptome suggest that heat shock proteins extend lifespan. Experimental Gerontology, 50, 34–39.
Selye, H. (1976). Stress without distress. In: Serban, G. (Ed.). Psychopathology of human adaptation. Springer, Boston. Pp. 137–146.
Selye, H. (1982). Stress bez distressa [Stress without distress]. Progress, Moscow (in Russian).
Sigida, R. (2009). Galofilnyye vidy zhuzhelitsy – kak indicatory zasolennykh biotopov Stepnoy zony Predkavkazia [Salt-loving species of carabids as indicators salted biotopes of Steppe zone of Ciscaucasia]. Vestnik Moskovskogo Gosudarstvennogo Oblastnogo Universiteta, 3, 49–56 (in Russian).
Slin'ko, V. O., Brygadyrenko, V. V., & Pakhomov, O. Y. (2008). Morfologicheskaja izmenchivost' Bembidion varium (Carabidae, Coleoptera) v uslovijah antropogennogo vozdejstvija [Morphological variability of Bembidion varium (Carabidae, Coleoptera) in the conditions of anthropogenic pressure]. Proceedings of the National Academy of Sciences of Azerbaijan (Biological Sciences), 64(5–6), 200–206 (in Russian).
Sukhanova, M. J., Shumnaya, L. V., Grenback, L. G., Gruntenko, N. E., Khlebodarova, T. M., & Rauschenbach, I. Y. (1997). Tyrosine decarboxylase and dopa decarboxylase in Drosophila virilis under normal conditions and heat stress: Genetic and physiological aspects. Biochemical Genetics, 35(3–4), 91–103.
Sukhodolskaya, R. (2013). Intraspecific body size variation in ground beetles (Coleoptera, Carabidae) in urban – suburban – rural – natural gradient. Acta Biologica Universitatis Daugavpiliensis, 13(1), 121–128.
Sukhodolskaya, R. (2016). Intra-specific body size variation of ground beetles (Сoleoptera: Сarabidae) in latitudinal gradient. Periodicum Biologorum, 118(3), 273–280.
Sukhodolskaya, R. A., & Saveliev, A. A. (2014). Effects of ecological factors on size-related traits in the ground beetle Carabus granulatus L. (Coleoptera, Carabidae). Russian Journal of Ecology, 45(5), 414–420.
Tilly, A. S. (2012). Zhuki-zhuzhelitsy (Coleoptera, Carabidae) zasolennykh pochv stepnogo Zavolzhia Samarskoy oblasti [Carabidae (Coleoptera) of saline soils on the Steppe Zone of Samara region]. Izvestiya Samarskogo Nauchnogo Tsentra Rossiyskoy Akademii Nauk, 14(1), 125–131 (in Russian).
Trost, M. (2003). Die Laufkäferfauna des Flächennaturdenkmals “Salzstelle bei TeutschenthalBahnhof” im Süden Sachsen-Anhalts. Naturschutz im Land Sachsen-Anhalt, 40(1), 19–32.
Trost, M. (2006). Die historische und aktuelle Bestandssituation der halobionten und halophilen Laufkäferfauna (Coleoptera, Carabidae) im Gebiet der Mansfelder Seen westlich von Halle/Saale (Sachsen-Anhalt). Hercynia N. F., 39, 121–149.
Tseng, M., & Soleimani Pari, S. (2018). Body size explains interspecific variation in size-latitude relationships in geographically widespread beetle species. Ecological Entomology, 44(1), 151–156.
Weller, B., & Ganzhorn, J. U. (2004). Carabid beetle community composition, body size, and fluctuating asymmetry along an urban-rural gradient. Basic and Applied Ecology, 5(2), 193–201.
Zakharenko, L. P., Karpova, E. K., & Rauschenbach, I. Y. (2014). P-M hybrid dysgenesis affects juvenile hormone metabolism in Drosophila melanogaster females. Russian Journal of Genetics, 50(7), 772–774.
Zhuravel, N., Polchaninova, N., Lezhenina, I., Drovgalenko, O., & Putchkov, A. (2016). Preliminary survey of the ground-dwelling arthropods of the flood-plain meadows in the southeast of Poltava region (Ukraine). Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(3), 5–17.

Most read articles by the same author(s)

> >>