Physiological and biochemical reactions of Hordeum vulgare seedlings to the action of silver nanoparticles


  • N. O. Khromykh Oles Honchar Dnipropetrovsk National University
  • L. V. Shupranova Oles Honchar Dnipropetrovsk National University
  • Y. V. Lykholat Oles Honchar Dnipropetrovsk National University
  • V. S. Bil’chuk Oles Honchar Dnipropetrovsk National University
  • V. S. Fedenko Oles Honchar Dnipropetrovsk National University
  • L. V. Boguslavs’ka Oles Honchar Dnipropetrovsk National University
  • O. I. Borysova Oles Honchar Dnipropetrovsk National University
Keywords: barley, peroxidase, glutathione, glutathione reductase, glutathione-S-transferase, nanosilver

Abstract

Morphometrical indexes, and spectrophotometrically measured protein and glutathione (GSH, GSSG) contents and activity of peroxidase (POD, EC 1.11.1.7), glutathione-reductase (GR, EC 1.6.4.2) and glutathione S-transferase (GST, EС 2.5.1.18) were examined in Hordeum vulgare L. seedlings after 0.01 and 0.1 mg/l AgNPs treatment during 24 h. We tested the hypothesis that the action of nanoparticles has a stressful effect on the physiological and biochemical processes of seedlings. Growth of roots was inhibited and fresh weight decreased by 29% and 21% under low and high concentrations respectively. Conversely, leaf growth was intensified, and leaf length (16% and 18%) and fresh weight (35% and 44%) increased at low and high concentrations respectively. POD activity in roots increased by 26% and 7%, and decreased in leaves to 57% and 81% of control at low and high concentrations respectively. GSH content changed insignificantly, but GSSG content increased in roots (2 and 2.5-fold) and in leaves (13% and 30%) at both AgNPs concentrations. GSH/GSSG-ratio decreased in roots (1.9 and 2.6-fold) and in leaves (1.1 and 1.3-fold) at low and high concentrations respectively. GR activity decreased at a concentration of 0.01 mg/l (7% in roots and 17% in leaves respectively) and increased at 0.1 mg/l (52% in roots and 6% in leaves). GST activity increased in leaves (52% and 78% at low and high concentrations) but decreased by 17% in roots under high concentration of nanosilver. Thus, the action of AgNPs on barley seedlings had a dose-dependent and organ-specific character. The various directions of changes in growth, metabolic processes and activity of antioxidant defense systems appear to be a stress response of barley seedlings to the impact of AgNPs, which underlines the necessity of detailed study of plant intracellular processes exposed to the action of nanomaterial. 

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Published
2015-07-03
Section
Articles

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