Influence of the slope altitude-associated microclimate and light conditions on the physiological and biochemical processes in leaves of coastal forest trees

  • N. A. Khromykh Oles Honchar Dnipropetrovsk National University
  • I. A. Ivan’ko Oles Honchar Dnipropetrovsk National University
  • I. M. Kovalenko Sumy National Agricultural University
  • Y. V. Lykholat Oles Honchar Dnipropetrovsk National University
  • A. A. Alexeyeva Oles Honchar Dnipropetrovsk National University
Keywords: Acer platanoides, Robinia pseudoacacia, slope, light, microclimate, chlorophyll, antioxidant enzymes


The problem of natural forests conservation is relevant in the Ukrainian steppe zone where the forest ecosystems develop in the unfavorable conditions of geographic mismatch; therefore, they suffer as a result of any environmental changes. The hypothesis of susceptibility of tree leaves’ metabolism even to slight changes of climatic parameters was tested. The study was conducted in more than 75 years-old natural mixed forest located on the right bank of the Samara river. The chlorophyll (Chl) content, and catalase (CAT), benzidine-peroxidase (BPOD) and guaiacol-peroxidase (GPOD) activities in leaves of autochthonous maple species (Acer platanoides L.) and adventive acacia species (Robinia pseudoacacia L.) were investigated on the lower (52 m above see level, a.s.l.), middle (74 m a.s.l.) and upper (96 m a.s.l.) coastal slope altitude. In maple leaves decreasing chlorophyll amount on the middle and upper plots (8.8% and 19.5% compared with the lower plot) together with Chla/Chlb ratio decrease (from 4.7 on the lower to 4.4 on the middle and 4.0 on the upper plots) was found. Chlorophyll content in acacia leaves decreased only on the upper plot (by 8.5% compared to the lower one), and Chla/Chlb ratio on the lower and upper plots reached 5.1. In maple leaves the increase in altitude was accompanied by the decrease in BPOD activity (by 26% and 63% on the middle and upper plots, accordingly), and GPOD and CAT (accordingly, by 31% and 37% on the upper plots compared to the lower plots). High correlation coefficients of light, temperature and humidity with activity of CAT (respectively, r = –0.98; r = –0.85; r = 0.83), BPOD (r = –0.96; r = –0.93; r = 0.90) and GPOD (r = –0.98; r = –0.82; r = 0.82) were estimated in A. platanoides leaves. Sharp decline in GPOD activity in R. pseudoacacia leaves was revealed on the upper plot (by 95% compared to the lower one), whereas BPOD activity increased by 47% on the middle plot but decreased by 74% on the upper one compared to the lower plot, and CAT activity on the middle and upper plots exceeded 4.3-fold and 1.8-fold the activity on the lower plot. In R. pseudoacacia leaves high correlation of light, temperature and humidity was found with GPOD activity (respectively, r = –0.99; r = –0.82; r = 0.82), whereas correlation with the light level was significant only for BPOD (r = –0.84), and no significant correlation was recorded for CAT activity. Results of the study confirm high sensibility of photosynthetic and antioxidant processes in leaves of both tree species to microclimate changes, which is defined as a conventional enhancement of aridity traits when moving upwards on the slope. It is suggested that the level of correlation between enzyme activity and microclimate and lighting parameters can serve as a marker of tree species adaptation to the slope altitude-associated changes of the local environmental factors in the natural forest. 


Alexeyev, V.A., 1975. Svetovoy rezhym lesa [Light regime of forest]. Nauka, Leningrad (in Russian).
Allison, S.D., Schultz, J.C., 2004. Differental activity of peroxidase isozymes in response to wounding, gypsy moth, and plant hormones in Nothern red oak (Quercus rubra L.). J. Chem. Ecol. 30(7), 1363–1379.
Bahuguna, R.N., Jagadish, K.S.V., 2015. Temperature regulation of plant phenological development. Environ. Exp. Bot. 111(3), 83–90. >>
Bel’gard, A.L., 1971. Stepnoe lesovedenye [Steppe Forestry]. Lesnaja promyshlennost, Moscow (in Russian).
Bussotti, F., Pollastrini, M., Holland, V., Bruggeman, W., 2015. Functional traits and adaptive capacity of European forests to climate change. Environ. Exp. Bot. 111(3), 91–113. >>
Caudle, K.L., Johnson, L.C., Baer, S.G. Maricle, B.R., 2014. A comparison of seasonal foliar chlorophyll change among ecotypes and cultivars of Andropogon gerardii (Poaceae) by using nondestructive and destructive methods. Photosynthetica 52(4), 511–518. >>
Dolgova, L.G., 2004. Aktivnost’ peroksidazy – pokazatel’ ustoychivosti rasteniy-introdutsentov v usloviyakh stepnoy zony Ukrainy [Peroxidase activity – an indicator of plant exotic species stability in the steppe zone of Ukraine]. Vìsn. Dnìpropetr. Unìv. Ser. Bìol. Ekol. 12(1), 38–42 (in Russian).
Goth, L., 1991. A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta 196, 143–152. >>
Gregory, R.P.F., 1966. A rapid assay for peroxidase activity. Biochem. J. 101(3), 582–583.
Grytsan, Y.I., 2000. Ekologichni osnovy peretvoryuyuchogo vplyvu lisovoy roslynnosty na stepove seredovysche [Ecological bases transformative impact of forest vegetation on steppe environment]. Dnipropetrovsk Univ. Press, Dnipropetrovsk (in Ukrainian).
Guo, B., Dai, S., Wang, R., Guo, J., Ding, Y., Xu, Y., 2015. Combined effects of elevated CO2-contamibated soil on the growth, gas exchange, antioxidant defense, and Cd accumulation of poplars and willows. Environ. Exp. Bot. 115(10), 1–10.
Harfouche, A., Meilan, R., Altman, A., 2014. Molecular and physiological responses to abiotic stresses in forest trees and their relevance to tree improvement. Tree Physiol. 34(11), 1181–1198.
Ivan’ko I.A., 2009. Znachennya typu svitlovoy struktury pry formuvanny shtuchnykh lisovukh biogeostenoziv u stepu [Significance of light structure in formation of planted forest ecosystems in steppe]. Pytannya lisovogo stepoznavstva ta lisovoy rekultivatsyi zemel 38, 59–64 (in Ukrainian).
Ivan’ko, I.A., 2008. Effekt melanizatsyi iskusstvennykh nasazhdeny kak faktor ykh ustoychivosti v stepnoy zone [Melanization effect of plantations as a factor in their sustainability in the steppe zone]. Ecology and Noospherology 19(3–4), 181–184 (in Russian).
James, S.A., Bell, D.T., 2000. Influence of light availability on leaf structure and growth of two Eucalyptus globulus ssp. globulus provenances. Tree Physiol. 20, 1007–1018. >>
Khromykh, N.O., Bilchuk, V.S., Rossykhina-Galycha, G.S., Vinnychenko, O.M., 2014. Sezonna dynamika antyoxidantnykh protsessiv u lystkah Acer negundo za diy polyutantiv [Seasonal dynamics of antioxidative processes in Acer negundo leaves under pollutant action]. Vìsn. Dnìpropetr. Unìv. Ser. Bìol. Ekol. 22(1), 71–76 (in Ukrainian).
Lee, B.-R., Kim, K.-Y., Jung, W.-J., Avice, J.-C., Ourry, A., Kim, T.-H., 2007. Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover (Trifolium repens L.). J. Exp. Bot. 58(6), 1271–1279. >>
Linder, M., Fitzgerald, J.B., Zimmermann, N.E., Reyer, C., Delzon, S., van der Maaten, E., Schelhass, M.-J., Lasch, P., Eggers, J., van der Maaten-Theunissen, M., Suckow, F., Promas, A., Poulter, B., Hanewinkel, M., 2014. Climate change and European forests: What do we know, what are the uncertainties, and what are the implications for forest management? J. Environ. Manage. 146(12), 69–83.
Luna, C.M., Pastori, G.M., Driscoll, S., Grotan, K., Bernard, S., Foyer, C.H., 2005. Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. J. Exp. Bot. 56(411), 417–423. >>
Morales, M., Garcia, Q.S., Munne-Bosch, S., 2015. Ecophysiological response to seasonal variations in water availability in the arborescent, endemic plant Vellozia gigantea. Tree Physiol. 35(3), 253–265.
Mund, M., Kutsch, W.L., Wirth, C., Kahl, T., Knohl, A., Skomarkova, M.V., Schulze, E.D., 2010. The influence of climate and fructification on the inter-annual variability of steam growth and net primary productivity in an old-growth, mixed beech forest. Tree Physiol. 30(6), 689–704.
Pavlov, I.N., 2004. Vliyanye vybrosov aluminievogo zavoda na soderzhanye chlorophilla v list’yakh depev’ev i kustarnykov [The impact of aluminum factory missions on chlorophyll content of trees and shrubs]. Sbornyk statey po materialam Vserossiyskoy nauch.-prakt. konferentsyi, Krasnoyarsk, 1, 164–170 (in Russian).
Queval, G., Thominet, D., Vanacker, H., Miginiac-Maslow, M., Gakiere, B., Noctor, G., 2009. H2O2-activated up-regulation of glutathione in Arabidopsis involves induction of genes encoding enzymes involved in cysteine synthesis in the chloroplasts. Mol. Plant 2(2), 344–356. >>
Rajsnerova, P., Klem, K., Holub, P., Novotna, K., Vecerova, K., Kozacikova, M., Rivas-Ubach, A., Sardans, J., Marek, M.-V., Penuelas, J., Urban, O., 2015. Morphological, biochemical and physiological traits of upper and lower canopy leaves European beech tends to converge with increasing altitude. Tree Physiol. 35(1), 47–60. >>
Ramirez-Valiente, J.A., Koehler, K., Cavender-Bares, J., 2015. Climatic origins predict variations in photoprotective leaf pigments in response to drought and law temperature in live oaks (Quercus series virentes). Tree Physiol. 35(1), 521–534.
Ranieri, A., Castagna, A., Baldam, B., Soldatini, G.F., 2001. Iron deficiency differently affects peroxidase isoforms in sunflower. J. Exp. Bot. 52(354), 25–35. >>
Ranieri, A., Castagna, A., Lorenzini, G., Soldatini, G.F., 1997. Changes in thylakoid protein patterns and antioxidant levels in two wheat cultivars with different sensitivity to sulphur dioxide. Environ. Exp. Bot. 37, 125–135.
Rogozhyn, V.V., 2004. peroxidaza kak komponent antioxidantnoy sistemy zhyvykh organizmov [Peroxidase as a component of antioxidative system of living organisms]. Giord, S.-Peterburg (in Russian).
Sperlich, D., Chang, C.T., Penuelas, J., Gracia, C., Sabate, S., 2015. Seasonal variability of foliar photosynthetic and morphological traits and drought impacts in a Mediterranean mixed forest. Tree Physiol. 35(5), 501–520. >>
Talbi, S., Romero-Puertas, M.S., Hernandez, A., Terron, L., Ferchichi, A., Sandalio, L.M., 2015. Drought tolerance in a Saharian plant Oudneya africana: Role of antioxidant defences. Environ. Exp. Bot. 111(3), 114–126. >>
Tikhonov, A.N., 1999. Regulyatsiya svetovykh i temnovykh stadiy photosynteza [Regulation of light and dark phases of photosynthesis]. Sorosovsky Obscheobrazovatelny Zhurnal 11, 8–15 (in Russian).
Tkach, V.P., 2012. Lisy ta lisystist v Ukrayni: Suchasny stan i perspektyvy rozvytku [Forests and forest cover in Ukraine: Current state and prospects for development]. Ukrayns’ky Geografichny Zhurnal 2, 49–55 (in Ukrainian).
Wintermans, J.F.G.M., De Mots, A., 1965. Spectrophotometric characteristics of chlorophyll a and b and their phaeophytins in etanol. Biochim. Biophys. Acta 109(2), 448–453.

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