Microbiota of the rhizosphere zone of Calamagrostis epigeios from a coal mine waste dump

  • N. P. Meniv Ivan Franko National University of Lviv
  • O. D. Maslovska Ivan Franko National University of Lviv
  • S. Y. Komplikevych Ivan Franko National University of Lviv
  • S. O. Hnatush Ivan Franko National University of Lviv
Keywords: recultivation; coal mine dumps; stages of succession; bacteria of nitrogen cycle; bacteria of sulfur cycle; cellulose-degrading microorganisms; pedotrophic microorganisms.

Abstract

The microbiota plays an important role in the processes of plant overgrowth of coal mine waste dumps, enabling the transformation of numerous compounds into forms available to plants. The overgrowth of coal mine dumps is influenced by many factors. Pioneers are plant species that have a wide ecological and phytocenotic amplitude. Calamagrostis epigeios (L.) Roth. occupies a special place among them. The composition of the microbiota of the rhizosphere zone of C. epigeios was studied in relation to the age of the plants and the stage of the succession of the “Vizeyska” mine dump (Ukraine). It was established and confirmed as a result of two-factor variance analysis that the growth phase of C. epigeios and the stage of the succession of coal mine waste dumps have different effects on the number of microorganisms from the rhizosphere zone of plants. The number of pedotrophic microorganisms, microorganisms that metabolize nitrogen of organic compounds, cellulose-degrading microorganisms, and microscopic fungi depended more on the age of C. epigeios, and not on the stage of the succession of the studied area. The number of chemolithotrophic bacteria, particularly thiobacteria, decreased with the change of the growth phase of C. epigeios. The number of acidophobic thiobacteria depended more on the stage of succession, and the number of acidophilic thiobacteria depended more on the age of the C. epigeios. The number of microorganisms that metabolize nitrogen of organic compounds, oligonitrophilic microorganisms and microorganisms that metabolize nitrogen of inorganic compounds in the samples of tailings from the area with grasses and perennials and from the area with grasses, shrubs, and sun-loving trees was higher, compared to the number of these groups of microorganisms in the control and changed with the change in the growth phase of C. epigeios. The number of microorganisms that metabolize nitrogen of organic compounds, oligonitrophilic microrganisms and microorganisms that metabolize nitrogen of inorganic compounds was the highest in the samples from the area with grasses, shrubs, and sun-loving trees during the adult growth phase of C. epigeios. In the area where C. epigeios dominated within the vegetation, the highest number of microorganisms that metabolize nitrogen of organic compounds was also during the adult phase of C. epigeios, and the number of bacteria that assimilate mineral forms of nitrogen and oligonitrophilic microorganisms was the highest during the sub-adult stage. The index of pedotrophicity is higher in the samples taken in the area where C. epigeios prevails among other herbaceous plants, and where in the tree layer there are Betula pendula, Populus tremula with an admixture of Pinus sylvestris. Pedotrophicity indices which were calculated for these samples do not depend on the growth stage of C. epigeios and are higher than for the control area. Immobilization-mobilization of nitrogen indices in samples of tailings from the area with grasses and perennials and from the area with grasses, shrubs, and sun-loving trees ranged from 1.94 to 3.52 and were higher compared to the control site.

References

Andreyuk, K. I., Iutyns’ka, H. O., Antypchuk, A. F., & Valahurova, O. V. (2001). Funktsionuvannya mikrobnykh tsenoziv gruntu v umovakh antropohennoho navantazhennya [Functioning of soil microbial coenoses under conditions of anthropogenic load]. Oberehy, Kyiv (in Ukrainian).

Baranov, V. I. (2008). Ekolohichnyy opys porodnoho vidvalu vuhil’nykh shakht TSZF ZAT “L’vivsystemenerho” yak ob’yekta dlya ozelenennya [Ecological scope of rock dump coal mines CCM CJ-SC “Lvivsystemergo” as subject to plant trees and grass]. Visnyk of Lviv University, Biological Series, 46, 172–178 (in Ukrainian).

Bashutska, U. B. (2006). Suktsesiyi roslynnosti porodnykh vidvaliv shakht Chervonohrads’koho hirnychopromyslovoho rayonu [Vegetation successions of waste dumps of mines of the Chervonograd mining district]. Ukrainian National Forestry University, Lviv (in Ukrainian).

Beshley, S. V. (2011). Osoblyvosti nahromadzhennya nadzemnoyi fitomasy Calamagrostis epigeios (L.) Roth u suktsesiynykh uhrupovannyakh na vidvalakh vuhil’nykh shakht Chervonohrads’koho hirnychopromyslovoho rayonu (L’vivs’ka oblast’) [Accumulation of Calamagrostis epigeios (L.) Roth. aboveground phytomass at the grass and wood-grass stages of plant succession on coal mines dumps of Chervonograd mining area (Lviv Region)]. Scientific Principles of Biodiversity Conservation, 2(9), 23–32 (in Ukrainian).

Beshley, S. V., Baranov, V. I., & Mykiyevych, I. M. (2010). Zmina substrativ vidvaliv porody Chervonohrads’koho hirnychopromyslovoho rayonu pry zarostanni kunychnykom nazemnym (Calamagrostis epigeios (L.) Roth) [Сhanges of the substrates on rock dumps of the Сhervonograd industrial coal-mining region at overgrowing with Calamagrostis epigeios (L.) Roth.]. Biolohichni Studii, 4(2), 75–82 (in Ukrainian).

Blayda, I. A., Vasyleva, T. V., Chitrich, V. F., Vasyleva, N. Y., Dgambek, O. I., & Dgambek, O. A. (2016). Fiziko-khimichna ta mikrobiolohichna khakterystyka porodnykh vidvaliv zbahachennya vuhillya [Physicochemical and microbiological characteristics of the waste dumps after coal enrichment]. Microbiology and Biotechnology, 2, 75–89 (in Ukrainian).

Boldy, R., Santini, T., Annandale, M., Erskine, P. D., & Sonter, L. J. (2021). Understanding the impacts of mining on ecosystem services through a systematic review. The Extractive Industries and Society, 8(1), 457–466.

Bulyhin, S., & Tonkha, O. (2018). Biological evaluation of the rationality of soil usage in agriculture. Agricultural Science and Practice, 5(1), 23–29.

Diakiv, S. V., Hnatush, S. O., & Halushka, А. А. (2017) Fizioloho-biokhimichni vlastyvosti Desulfuromusa sp. SV30, vydilenykh iz porodnykh vidvaliv vuhil’nykh shakht [Physiological and biochemical characteristics of Desulfuromusa sp. SV30, isolated from coal pits waste heaps]. Mikrobiolohichnyi Zhurnal, 79(5), 80–90 (in Ukrainian).

Diakiv, S., Hnatush, S., & Meniv, N. (2016). Tselyulazna aktyvnist’ porodnykh vidvaliv vuhil’nykh shakht Chervonohrads’koho hirnychopromyslovoho rayonu [Cellulase activity of the coal pits’ waste heaps of Chervonograd mining region]. Visnyk of Lviv University, Biological Series, 74, 150–160 (in Ukrainian).

Etesami, H., & Beattie, G. A. (2018). Mining halophytes for plant growth-promoting halotolerant bacteria to enhance the salinity tolerance of non-halophytic crops. Frontiers in Microbiology, 9, 148.

Gazitúa, M. C., Morgante, V., Poupin, M. J., Ledger, T., Rodríguez-Valdecantos, G., Herrera, C., González-Chávez, M. C., Ginocchio, R., & González, B. (2021). The microbial community from the early-plant colonizer (Baccharis linearis) is required for plant establishment on copper mine tailings. Scientific Reports, 11(1), 1–16.

Gupta, S., & Pandey, S. (2019). ACC deaminase producing bacteria with multifarious plant growth promoting traits alleviates salinity stress in French bean (Phaseolus vulgaris) plants. Frontiers in Microbiology, 10, 1506.

Harfoot, M. B., Tittensor, D. P., Knight, S., Arnell, A. P., Blyth, S., Brooks, S., Butchart, S. H. M., Hutton, J., Jones, M. I., Kapos, V., Scharlemann, J. P. W., & Burgess, N. D. (2018). Present and future biodiversity risks from fossil fuel exploitation. Conservation Letters, 11(4), e12448.

Hudz’, S. P., Hnatush, S. O., Yavorska, H. V., Bilinska, I. S., & Borsukevych, B. M. (2014). Praktykum z mikrobiolohii [Guidebook on microbiology]. Vydavnychyy Tsentr L’vivs’koho Natsional’noho Universytetu imeni Ivana Franka, Lviv (in Ukrainian).

Kompała-Bąba, A., Bierza, W., Błońska, A., Sierka, E., Magurno, F., Chmura, D., Besenyei, L., Radosz, Ł., & Woźniak, G. (2019). Vegetation diversity on coal mine spoil heaps – how important is the texture of the soil substrate? Biologia, 74(4), 419–436.

Kompała-Bąba, A., Bierza, W., Sierka, E., Błońska, A., Besenyei, L., & Woźniak, G. (2021a). The role of plants and soil properties in the enzyme activities of substrates on hard coal mine spoil heaps. Scientific Reports, 11(1), 1–13.

Kompała‐Bąba, A., Sierka, E., Bierza, W., Bąba, W., Błońska, A., & Woźniak, G. (2021b). Eco‐physiological responses of Calamagrostis epigejos L (Roth.) and Solidago gigantea Aition to complex environmental stresses in coal‐mine spoil heaps. Land Degradation and Development, 32(18), 5427–5442.

Kompała-Bąba, A., Sierka, E., Dyderski, M. K., Bierza, W., Magurno, F., Besenyei, L., Błońskaa, A., Ryśa, K., Jagodziński, A. M., & Woźniak, G. (2020). Do the dominant plant species impact the substrate and vegetation composition of post-coal mining spoil heaps? Ecological Engineering, 143, 105685.

Kumar, A., & Verma, J. P. (2018). Does plant – microbe interaction confer stress tolerance in plants: A review? Microbiological Research, 207, 41–52.

Kuzmishyna-Diakiv, S., & Hnatush, S. (2015). Microbiota of the coal pits waste heaps. OmniScriptum GmbH & Co. KG, Lambert Academic Publishing, Saarbrücken.

Li, Y., Gao, P., Sun, X., Li, B., Guo, L., Yang, R., Su, X., Gao, W., Xu, Z., Yan, G., Wang, Q., & Sun, W. (2022a). Primary succession changes the composition and functioning of the protist community on mine tailings, especially phototrophic protists. American Chemical Society Environmental Author University.

Li, Y., Guo, L., Häggblom, M. M., Yang, R., Li, M., Sun, X., Chen, Z., Li, F., Su, X., Yan, G., Xiao, E., Zhang, H., & Sun, W. (2022b). Serratia spp. are responsible for nitrogen fixation fueled by As (III) oxidation, a novel biogeochemical process identified in mine tailings. Environmental Science and Technology, 56(3), 2033–2043.

Li, Y., Zhang, M., Xu, R., Lin, H., Sun, X., Xu, F., Gao, P., Kong, T., Xiao, E., Yang, N., & Sun, W. (2021). Arsenic and antimony co-contamination influences on soil microbial community composition and functions: Relevance to arsenic resistance and carbon, nitrogen, and sulfur cycling. Environment International, 153, 106522.

Mghazli, N., Sbabou, L., Hakkou, R., Ouhammou, A., El Adnani, M., & Bruneel, O. (2021). Description of microbial communities of phosphate mine wastes in Morocco, a semi-arid climate, using high-throughput sequencing and functional prediction. Frontiers in Microbiology, 12, 666936.

Oyetibo, G. O., Enahoro, J. A., Ikwubuzo, C. A., & Ukwuoma, C. S. (2021). Microbiome of highly polluted coal mine drainage from Onyeama, Nigeria, and its potential for sequestrating toxic heavy metals. Scientific Reports, 11(1), 1–15.

Poberezhsky, A., Buchynska, I., Shevchuk, O., & Mukan, T. (2019). Hirnychovydobuvnyy kompleks L’vivs’ko-Volyns’koho vuhil’noho baseynu ta yoho vplyv na ekosystemu rehionu [Mining complex of the Lviv-Volyn coal basin and its impact on the ecosystem of the region]. Geology and Geochemistry of Combustible Minerals, 180, 52–59 (in Ukrainian).

Popovych, V. V., Mysak, R. I., & Brunec, K. S. (2011). Pryrodna fitomelioratsiya vuhil’nykh vidvaliv [Natural phytomelioration of coal dumps]. Scientific Bulletin of Ukrainian National Forestry University, 21(4), 127–131 (in Ukrainian).

Rud’ko, H. I., Ivanov, Y. A., & Koval’chuk, I. P. (2019). Hirnychopromyslovi heosystemy Zakhidnoho rehionu Ukrayiny [Geosystems of the chemical industry in the Western region of Ukraine]. Bukrek, Kyiv, Chernivtsi (in Ukrainian).

Saphonova, A. S., & Reva, S. V. (2009). Zaselennya vyshchymy roslynamy zalizorudnykh vidvaliv Kryvbasu [Colonization by higher plants of iron – ore dumps of Krivyi Rig Basin]. Visnyk of Dnipropetrovsk University, Biology. Ecology, 17(2), 87–94 (in Ukrainian).

Singh, R. P., Ma, Y., & Shadan, A. (2022). Perspective of ACC-deaminase producing bacteria in stress agriculture. Journal of Biotechnology, 352, 36–46.

Sun, W., Xiao, E., Häggblom, M., Krumins, V., Dong, Y., Sun, X., Li, F., Wang, Q., Li, B., & Yan, B. (2018). Bacterial survival strategies in an alkaline tailing site and the physiological mechanisms of dominant phylotypes as revealed by metagenomic analyses. Environmental Science and Technology, 52(22), 13370–13380.

Sun, W., Xiao, E., Krumins, V., Dong, Y., Li, B., Deng, J., Wang, Q., Xiao, T., & Liu, J. (2019). Comparative analyses of the microbial communities inhabiting coal mining waste dump and an adjacent acid mine drainage creek. Microbial Ecology, 78(3), 651–664.

Sun, X., Kong, T., Häggblom, M. M., Kolton, M., Li, F., Dong, Y., Huang, Y., Li, B., & Sun, W. (2020a). Chemolithoautotropic diazotrophy dominates the nitrogen fixation process in mine tailings. Environmental Science and Technology, 54(10), 6082–6093.

Sun, X., Li, B., Han, F., Xiao, E., Xiao, T., & Sun, W. (2019). Impacts of arsenic and antimony co-contamination on sedimentary microbial communities in rivers with different pollution gradients. Microbial Ecology, 78(3), 589–602.

Sun, X., Qiu, L., Kolton, M., Häggblom, M., Xu, R., Kong, T., Gao, P., Li, B., Jiang, C., & Sun, W. (2020b). VV reduction by Polaromonas spp. in vanadium mine tailings. Environmental Science and Technology, 54(22), 14442–14454.

Volkohon, V. V., Nadkernychna, O. V., Tokmakova, L. M., Mel’nychuk, T. M., Chaykovs’ka, L. O., Nadkernychnyy, S. P., Sherstoboyev, M. K., Kozar, S. F., Kopylov, Y. P., Krutylo, D. V., Parkhomenko, T. Y., Kamyenyeva, I. O., Adamchuk-Chala, N. I., Kovalevs’ka, T. M., Didovych, S. V., Volkohon, K. I., Pyshchur, M. I., Volkohon, M. V., Dimova, S. B., & Komok, M. S. (2010). Eksperymental’na gruntova mikrobiolohiya [Experimental soil microbiology]. Ahrarna Nauka, Kyiv (in Ukrainian).

Wang, L., Ji, B., Hu, Y., Liu, R., & Sun, W. (2017). A review on in situ phytoremediation of mine tailings. Chemosphere, 184, 594–600.

Yamborko, N. A., Dimova, M. I., & Iutynska, G. O. (2019). Influence of hechachlorobenzene on microbiota of chernozem soil. Mikrobiolohichnyi Zhurnal, 81(5), 27–35.

Published
2022-07-27
Section
Articles

Most read articles by the same author(s)